Identification of Human Papillomavirus Type 18 (HPV 18) E7 Gene from Cervical Cancer Patients
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Abstract
One oncoprotein that is always expressed by HPV 18 in cervical cancer is the E7 gene. Persistent infection by the human papillomavirus (HPV) high risk is a major etiologic factor for cervical cancer and expression of E7 Oncoproteins suggested to be a potential marker for tumor progression. E7 will affect the activity of tumor suppressor pRB and bind and activate cyclin complexes that can cause cervical cancer. In this study will be identification of the isolates HPV18 E7 gene of patients with cervical cancer derived from M. Jamil Hospital in Padang, West Sumatra and Arifin Ahmad, Pekanbaru, Riau. The identification process was conducted using Polymerase Chain Reaction (PCR) using primers specific for HPV18 E7 gene. Of the fifteen isolates HPV18 were amplified with specific primers HPV18 E7 gene, obtained ten E7 genes identified positive samples (66.6%), while five other samples were not identified. Infection cancer in patients with cervical cancer that come from the M. Jamil Padang and Arifin Ahmad, Pekanbaru, Riau hospitals mostly caused by HPV18 E7 gene so that it can be used as a potential marker for tumor progression for patients with cervical cancer in West Sumatra and Riau in particular and Indonesia in general.
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