Determination of berberine content of ethanol extract of root and stem of “sekunyit” (Fibraurea tinctoria Lour) using high performance liquid chromatography (HPLC) method

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Published: May 29, 2017
DOI: https://doi.org/10.29208/jsfk.2017.3.2.84
Keywords:
berberine, Fibraurea tinctoria, HPLC.

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Rahayu Utami
STIFAR Riau
Armon Fernando
STIFAR Riau
Indah Puspita Sari
STIFAR Riau
Mustika Furi
STIFAR Riau

Abstract

Determination of berberine content of ethanol extract of root and stem of “sekunyit†(Fibraurea tinctoria Lour) has been conducted. “Sekunyit†is one of medicinal plant that has been used to treat several diseases traditionally. Its root and stem could relieve jaundice, diarrhea, conjunctivitis as well as antidiabetic agent. Based on previous study, it is known that Fibraurea tinctoria contains isoquinoline alkaloid, berberine. This present study aims to determine berberine content which was done by HPLC (High Performance Liquid Chromatography) method using C-18 reverse phase column, methanol : phosphate buffer (pH 6,8) as its mobile phase with flow rate of 1 ml/min and UV detector. The analysis was performed at wavelength 346 nm. The result showed that the ethanol extract contains 25.8% of berberine

Article Details

How to Cite
Utami, R., Fernando, A., Sari, I. P., & Furi, M. (2017). Determination of berberine content of ethanol extract of root and stem of “sekunyit” (Fibraurea tinctoria Lour) using high performance liquid chromatography (HPLC) method. Jurnal Sains Farmasi & Klinis, 3(2), 115–119. https://doi.org/10.29208/jsfk.2017.3.2.84
Issue
Vol. 3 No. 2 (2017)
Section
Research Articles

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Author Biography

Rahayu Utami, STIFAR Riau

Dosen Tetap Yayasan Universitas Riau, Sebagai Pembantu Ketua III, Dosen Penata Muda Tk.I, golongan IIIb, dengan jabatan fungsional lektor.

References

Priyadi, H., Takao, G., Rahmawati, I., Supriyanto, B., Nursal, W I., and Rahman, I. (2010). Five Hundred Plant Species in Gunung Halimun Salak National Park, West Java : A Checklist including Sundanese Names, Distribution and Use. Bogor, Indonesia : Cifor. Page 91-92.

Anonim. (2012). Riset Khusus Eksplorasi Pengetahuan Lokal Etnomedisin dan Tumbuhan Obatdi Indonesia Berbasis Komunitas, Laporan Provinsi Riau dan Kepulauan Riau. Pekanbaru : Lembaga Penelitian Universitas Riau.

Su, C.-R., Chen, Y.-F., Liou, M.-J., Tsai, H.-Y., Chang, W.-S. And Wu, T.-S. (2008). Anti-inflammatory activities of furanoditerpenoids and other constituents from Fibraurea tinctoria. Bioorganic & Medicinal Chemistry, 16, 9603-9609.

Su, C.-R., Ueng, Y.-F., Dung, N. X., Vijaya Bhaskar Reddy, M. And Wu, T.-S. (2007). Cytochrome P3A4 Inhibitors and Other Constituents of Fibraurea tinctoria. Journal of Natural Products, 70, 1930-1933.

Keawpradub, N., Dej-adisai, S. And Yuenyongsawad, S. (2005). Antioxidant and cytotoxic activities of Thai medicinal plants named Khaminkhruea: Arcangelisia flava, Coscinium blumeanum and Fibraurea tinctoria. Songklanakarin Journal of Science and Technology, 27, 455-467.

Sharma, A. And Batra, A. (2013). Berberine a novel anti-diabetic drug. International Journal of Research and Review in Pharmacy and Applied Sciences, 3(2), 216-230.

Utami, R., Sandi, N.H., Hasti, S. And Delvia, S. (2015). Uji Aktivitas Antidiabetes Ekstrak Etanol dari Akar dan Batang Tumbuhan Sekunyit (Fibraurea Tinctoria Lour). Jurnal Farmasi Indonesia, 7(4), 216-222.

Srinivasan, G. V., Unnikrishnan, K. P., Rema Shree, A. B., and Balachandran, I. (2008). HPLC Estimation of Berberine in Tinospora cordifolia and Tinospora sinensis. Indian Journal of Pharmaceutical Sciences, 70(1), 96-99.

Tsai, P. L., and Tsai, T. H. (2002). Hplc Determination Of Berberine In Medicinal Herbs And A Related Traditional Chinese Medicine. University of Malaya : Analytical Letter.

Rohman, A. (2007). Kimia Farmasi Analisis, Yogyakarta : Penerbit UGM.