Potential of Red Spinach Leaves (Amaranthus tricolor L. ) Ethanolic Extract Standardized as Antihyperlipidemia: in Vivo Study Based on LDL (Low Density Lipoprotein) Parameter
Article Sidebar
Main Article Content
Abstract
This study aims to determine the potential for curative therapy ethanolic extract of red spinach leaves (Amaranthus tricolor L.) standardized to decrease LDL levels in vivo. Animal model used in this study were 30 male Wistar rats aged 2-3 months were randomly divided into 6 groups include normal control, negative control, positive control and three doses ratings eksktrak. Induction of hyperlipidemia using poloxamer on day 1st and propylthiouracil on day 5th to day 18th. The positive control groups were given by simvastatin and the treatment group were given three doses of the extract variations on each - each group ie 200 mg / kg, 400 mg / kg, and 800 mg / kg rat. Ethanolic extract of red spinach leaves that are used have been through a standardized test based on specific parameters and non-specific. Determination of plasma LDL levels was done 3 times, day 0 (baseline), day 4th (after induction process) and day 19th (after treatment). The results obtained show the ethanolic extract of red amaranth (Amaranthus tricolor L.) standardized at a dose of 800 mg / kg body weight can lower LDL levels statistically significant (p <0.05) compared with normal and negative group. It can be concluded that the ethanolic extract red spinach leaves standardized has activity as a curative therapy for hyperlipidemia.
Â
Article Details
The authors retain the copyright and grant the journal the right of first publication simultaneously under the Creative Commons Attribution License. This license allows others to share the work with proper acknowledgment of authorship and initial publication in this journal. Authors are permitted and encouraged to deposit their articles in institutional repositories, on their personal websites, or in other online repositories after the article has been published in JSFK.
References
Talbert, R.L. (2005). Hyperlipidemia, In Dipiro, J, T., Talbert R, L., Yee, G, C., Matzke, G. R., Wells, B.G., Posey, L. M., (Eds), Pharmacology, A Pathophisiologyc Approach, 6th Edition, McGraw-Hill Medical Publishing Division, USA.
Hatma, R.D. (2011). Sosial Determinan Dan Faktor Risiko Kardiovaskular Heart Disease in Dyslipidemic Patients: Results from a survey in 13 cities in Indonesia, Med. J. Indones, 10: 42-4
Keputusan Menteri Kesehatan Republik Indonesia Nomor 261/MENKES/SK/IV/2009, Farmakope Herbal Indonesia Edisi Pertama Kesehatan, 2: 15-20
Rustanto, T.N. (2013). Efek Ekstrak Metanol Daun Bayam (Amaranthus sp) terhadap Kadar Kolesterol Tikus (Rattus norvegicus galur Wistar) yang diberi Diet Aterogenik. Skripsi, Universitas Brawijaya: Malang.
Aneja, S., Vats, M., Sardana, S., Aggarwal, S. (2011). Pharmacognostic Evaluation and Phytochemical Studies On The Roots of Amaranthus Tricolor (Linn.). International Journal of Pharmaceutical Science and Research. Vol. 2 (9): 2336
Desai PV. (2011). Evaluation of The Hematological, Hypoglycemic, Hypolipidemic and Antioxidant Properties of Amaranthus Tricolor Leaf Extract in rat. Tropical Journal of Pharmaceutical Research. Nigeria; 10 (05). p.600-601
Agustuningsih. Wildan A. Mindaningsih. (2010). Optimasi Cairan Penyari Pada Pembuatan Ekstrak Daun Pandan Wangi (Pandanus amaryllifous Roxb) Secara Maserasi Terhadap Kadar Fenolik dan Flavonoid Total. Momentum ; 6 (2). p.38
Departemen Kesehatan Republik Indonesia. (1989) Materia Medika Indonesia Jilid V & VI. Jakarta: Departemen Kesehatan Republik Indonesia.
Departemen Kesehatan Republik Indonesia. (2000). Parameter Standar umum Ekstrak Tumbuhan Obat, Jakarta: Departemen Kesehatan Republik Indonesia
Badan Standarisasi Nasional. (2003). Batas Maksimum Cemaran Mikroba dalam Pangan, Badan Standarisasi Nasional, Jakarta. 2003.
Widaningrum. Miskiyah. Suismono. (2007). Bahaya Kontaminasi Logam Berat dalam Sayuran dan Alternatif Pencegahan Cemarannya, Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian. Buletin Teknologi Pascapanen.; 3. p.18 – 25.
Herwiyarirasanta., BA, Eduardus. (2010). Effect of Black Soybean Extract Supplementation in Low Density Lipoprotein Level of Rats (Rattus norvegicus) With High Fat Diet. Science Article Universitas Airlangga. Surabaya
Wasan, K,M.,Subramanian, R., Kwong, M. (2010). Poloxamer 407-mediated Alterations in The Activities of Enzyme Regulating Lipid Metabolism in Rats, J Pharm Pharmaceut Sci, 2010 ; p.189.
Leon C. Wasan KM. Sachs BK. Johnston TP. (2006). Acute P-407 Administration to Mice Causes Hypercholesterolemia by Inhibiting Cholesterogenesis and Down Regulating Low Density Lipoprotein Receptor Expressions, Pharmaceutical Research; 23 (7): 1597 – 1607.
Chaundary HR. Brocks DR. (2013). The Single Dose Poloxamer 407 Model of Hyperlipidemia; Systemic Effects on Lipids Assessed Using Pharmacokinetic Methods, and its Effect on Adipokines, Journal Pharm. Pharmaceut. Sci.; 16(1): 65-73
Marks DB. (2000) Metabolisme Kolesterol dan Lipoprotein Darah in Basic Medical Biochemistry: A clinical approach. Philadelphia, Pennsylvania. Brahm U.Pendit. Biokimia Kedokteran Dasar: Sebuah Pendekatan Klinis. Jakarta: EGC, 1996.
Alrejaie S. Abdulaziz AM. Ahmed MS. Alshabanah OA. Abouhashish HM. Ahmed MM. Alkhosaini KA. Hafez MM. (2013) Protective effect of rutin on the antioxidant genes expression in hypercholestrolemic male Westar rat, BMC Complementary and Alternative Medicine; 136 (13). p.4