Uji Aktivitas Antioksidan dan Daya Hambat Enzim Tirosinase Ekstrak Etanol Azolla filiculoides Lam.

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Published: Apr 30, 2021
DOI: https://doi.org/10.25077/jsfk.8.1.53-59.2021
Keywords:
aktivitas antioksidan, azolla filiculoides, 1, 1-diphenyl-2-picrylhydrazin, DPPH, tirosinase

Main Article Content

Bayu Febram Prasetyo
Fakultas Kedokteran Hewan Institut Pertanian Bogor

Abstract

Azolla (Azolla filiculoides Lam.) merupakan tanaman paku air dari famili Azollaceae. Azolla filiculoides digunakan sebagai pakan alami dengan ketersediaannya melimpah di alam yang belum termanfaatkan secara optimal. Tanaman ini dapat bersimbiosis dengan Cyanobacteria. Azolla memiliki senyawa bioaktif yang berfungsi sebagai antioksidan dan inhibitor tirosinase. Tujuan dari penelitian ini adalah untuk menguji aktivitas antioksidan menggunakan metode DPPH (2.2-difenil 1-pikrihidrasil ) dan daya hambat tirosinase pada ekstrak etanol Azolla filiculoides. Ekstraksi menggunakan metode maserasi dan pelarut etanol 96%. Hasil uji fitokimia menunjukkan bahwa ekstrak etanol Azolla mengandung senyawa flavonoid, tanin, saponin, steroid dan triterpenoid. Kandungan fitokimia tersebut berpotensi sebagai antioksidan dan inhibitor tirosinase, terutama senyawa flavonoid dan tanin. Uji aktivitas antioksidan menunjukkan bahwa ekstrak etanol Azolla filiculoides memiliki aktivitas antioksidan yang kuat dengan nilai IC50 sebesar 23.00 ppm. Uji aktivitas inhibisi tirosinase ekstrak azolla menunjukkan daya hambat yang lebih efektif pada aktivitas diphenolase (IC50 996.60 ppm) dibandingkan aktivitas monophenolase (IC50 1893.09 ppm).

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How to Cite
Prasetyo, B. F. (2021). Uji Aktivitas Antioksidan dan Daya Hambat Enzim Tirosinase Ekstrak Etanol Azolla filiculoides Lam. Jurnal Sains Farmasi & Klinis, 8(1), 53–59. https://doi.org/10.25077/jsfk.8.1.53-59.2021
Issue
Vol. 8 No. 1 (2021): J Sains Farm Klin 8(1), April 2021
Section
Research Articles
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