The effect of polymer amount on physical characteristics and in vitro release of diterpene lactone fraction of sambiloto-chitosan nanoparticles

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JSFK 7(2) Agustus 2020
PDF (Bahasa Indonesia)
Published: Aug 31, 2020
DOI: https://doi.org/10.25077/jsfk.7.2.99-106.2020
Keywords:
chitosan, Andrographis paniculata, sambiloto diterpene lactone fraction, ionic gelation, nanoparticles, drug release.

Main Article Content

Retno Sari
Fakultas Farmasi Universitas Airlangga
https://orcid.org/0000-0002-3391-1877
Abhimata Paramanandana
Fakultas Farmasi Universitas Airlangga
https://orcid.org/0000-0002-1990-9391
Dewi Isadiartuti
Fakultas Farmasi Universitas Airlangga
Annisa Maulidia Rahayyu
Fakultas Farmasi Universitas Airlangga

Abstract

The diterpene lactone fraction of Andrographis paniculata Nees (sambiloto) (FDTLS) main compound is andrographolide. Andrographolide has poor bioavailability, high lipophilicity, and low solubility. Entrapping poorly soluble substances into a hydrophilic polymer, such as chitosan, could improve their dissolution, bioavailability, and activity. The purposes of this study were to improve the FDTLS dissolution and to compare the effect of different amounts of chitosan on physical characteristics, entrapment efficiency, and FDTLS release from nanoparticles, which was made by the ionic gelation-spray drying method using tripolyphosphate as a crosslinker. The nanoparticles were prepared with different FDTLS-chitosan ratios = 1:2 (FK1), 1:2.5 (FK2) and 1:3 (FK3). The nanoparticles were evaluated for its morphology, physical state using differential thermal analysis (DTA), Fourier-transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), entrapment efficiency, and drug release. All the nanoparticles' formulas had a spherical shape and FK2 showed the smoothest surface with the highest enthalpy. FDTLS-chitosan nanoparticles diffractogram showed a more amorphous form. The FDTLS-chitosan nanoparticles entrapment efficiency was 29.01-32.69%. The FDTLS-chitosan nanoparticles showed an increased dissolution rate by 1.6 times compared to the FDTLS substance. The study concludes that the formation of nanoparticles using chitosan by ionic gelation-spray dry method could improve the dissolution of FDTLS.

Article Details

How to Cite
Sari, R., Paramanandana, A., Isadiartuti, D., & Rahayyu, A. M. (2020). The effect of polymer amount on physical characteristics and in vitro release of diterpene lactone fraction of sambiloto-chitosan nanoparticles. Jurnal Sains Farmasi & Klinis, 7(2), 99–106. https://doi.org/10.25077/jsfk.7.2.99-106.2020
Issue
Vol. 7 No. 2 (2020): J Sains Farm Klin 7(2), Agustus 2020
Section
Research Articles
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Author Biographies

Retno Sari, Fakultas Farmasi Universitas Airlangga

Departement Farmasetika

 

Abhimata Paramanandana, Fakultas Farmasi Universitas Airlangga

Departement Farmasetika

Dewi Isadiartuti, Fakultas Farmasi Universitas Airlangga

Departement Farmasetika

Annisa Maulidia Rahayyu, Fakultas Farmasi Universitas Airlangga

Departement Farmasetika

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