Identifikasi Mekanisme Molekuler Senyawa Bioaktif Peptida Laut sebagai Kandidat Inhibitor Angiotensin-I Converting Enzyme (ACE)

Taufik Muhammad Fakih; Mentari Luthfika Dewi

doi:10.25077/jsfk.7.1.76-82.2020

Identifikasi Mekanisme Molekuler Senyawa Bioaktif Peptida Laut sebagai Kandidat Inhibitor Angiotensin-I Converting Enzyme (ACE)

Taufik Muhammad Fakih, Mentari Luthfika Dewi

10.25077/jsfk.7.1.76-82.2020

Abstrak

Senyawa bioaktif peptida laut saat ini menjadi fokus penelitian karena memiliki sifat yang unik. Salah satu peran biologis penting dari senyawa peptida tersebut adalah sebagai agen antihipertensi terhadap aktivitas Angiotensin-I Converting Enzyme (ACE). Terdapat beberapa senyawa peptida yang telah terbukti mampu menghambat reseptor ACE, seperti senyawa peptida yang dihasilkan oleh teripang (Acaudina molpadioides), kerang biru (Mytilus edulis), dan ikan tuna (Thunnini). Dalam penelitian ini dilakukan identifikasi dan evaluasi terhadap interaksi yang terjadi antara senyawa peptida dengan reseptor ACE menggunakan motode penambatan molekuler berbasis protein-peptida. Sequencing senyawa peptida dimodelkan terlebih dahulu menggunakan server PEP-FOLD. Konformasi terbaik dipilih untuk dilakukan studi interaksi terhadap makromolekul reseptor ACE menggunakan software PatchDock. Interaksi yang terjadi diamati lebih lanjut menggunakan software BIOVIA Discovery Studio 2020. Berdasarkan hasil dari penambatan molekuler berbasis protein-peptida, senyawa peptida kerang biru dan ikan tuna memiliki afinitas yang baik terhadap reseptor ACE, yaitu dengan ACE score masing-masing adalah −391,62 kJ/mol dan −516,56 kJ/mol. Dengan demikian, senyawa bioaktif peptida laut tersebut diprediksi dapat dipilih sebagai kandidat inhibitor reseptor ACE berbasis peptida

Kata Kunci

antihipertensi; senyawa bioaktif peptida laut; inhibitor Angiotensin-I Converting Enzyme (ACE); pola penghambatan; penambatan molekuler berbasis protein-peptida.

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Referensi

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DOI: https://doi.org/10.25077/jsfk.7.1.76-82.2020