Effect of extraction methods on total phenol and flavonoid contents, antioxidant and antilipase activities of West Indian elm leaves (Guazuma ulmifolia)

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JSFK 7(2) Agustus 2020
PDF (Bahasa Indonesia)
Published: Aug 31, 2020
DOI: https://doi.org/10.25077/jsfk.7.2.143-150.2020
Keywords:
west indian elm, Guazuma ulmifolia, extraction methods, antioxidant, antilipase.

Main Article Content

Nuri Nuri
Fakultas Farmasi Universitas Jember
Endah Puspitasari
Fakultas Farmasi Universitas Jember
Mochammad Amrun Hidayat
Fakultas Farmasi Universitas Jember
Indah Yulia Ningsih
Fakultas Farmasi Universitas Jember
Bawon Triatmoko
Fakultas Farmasi Universitas Jember
Dewi Dianasari
Fakultas Farmasi Universitas Jember

Abstract

The leaf of Guazuma ulmifolia has been used traditionally for antiobesity. The activity of antiobesity was affected by the content of bioactive compounds. Extraction is the primary step to obtain bioactive compounds from plant material. The method and solvent used for extraction are crucial factors to produce extracts that have a high amount of active compounds. This study aims to determine the total phenolic and total flavonoids content from ethanolic extracts, water extract, and infusions of G. ulmifolia leaf and to evaluate the antioxidant and antilipase activity. Folin-Ciocalteu method was used to determine the phenolic content, while flavonoid content determination was done using aluminium chloride colorimetric assay. The antioxidant activity was done using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and the antilipase activity was quantified using p-nitrophenol release from p-nitrophenyl butyrate (p-NPB) substrate-colorimetric assay. The result of G. ulmifolia leaf extraction showed that the highest yield was obtained from water extraction (10.50%). Whereas, the ethanolic extract was showed the highest total phenolic content (67.761±1.811 mg GAE/g extract) and the highest total flavonoid content (124.643 ± 1.033 mg QE/g extract). The same extract also exhibited the highest antioxidant activity (IC50 = 6.544 ± 0.271 µg/mL) and antilipase activity (IC50 = 307.280 ± 21.430 µg/mL).

Article Details

How to Cite
Nuri, N., Puspitasari, E., Hidayat, M. A., Ningsih, I. Y., Triatmoko, B., & Dianasari, D. (2020). Effect of extraction methods on total phenol and flavonoid contents, antioxidant and antilipase activities of West Indian elm leaves (Guazuma ulmifolia). Jurnal Sains Farmasi & Klinis, 7(2), 143–150. https://doi.org/10.25077/jsfk.7.2.143-150.2020
Issue
Vol. 7 No. 2 (2020): J Sains Farm Klin 7(2), Agustus 2020
Section
Research Articles
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Author Biographies

Nuri Nuri, Fakultas Farmasi Universitas Jember

Departemen Biologi Farmasi

Endah Puspitasari, Fakultas Farmasi Universitas Jember

Departemen Biologi Farmasi

Mochammad Amrun Hidayat, Fakultas Farmasi Universitas Jember

Departemen Biologi Farmasi

Indah Yulia Ningsih, Fakultas Farmasi Universitas Jember

Departemen Biologi Farmasi

Bawon Triatmoko, Fakultas Farmasi Universitas Jember

Departemen Biologi Farmasi

Dewi Dianasari, Fakultas Farmasi Universitas Jember

Departemen Biologi Farmasi

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