Kekerabatan Genetik dan Indeks Resistensi Antibiotika Escherichia coli dari Air DAS Siak Provinsi Riau

Yuli Haryani, Emma Susanti, Hexsha Rizki Amelia

Abstrak


Waterborne-disease masih memberikan angka prevalensi yang tinggi terutama di negara sedang berkembang. Keberadaan E. coli di perairan memberikan indikasi adanya cemaran pathogen dari feses hewan ataupun manusia. Penelitian ini bertujuan untuk menganalisa kekerabatan dan indeks resistensi antimikroba dari E.coli yang telah diisolasi dari DAS Siak, sungai terbesar yang melewati Provinsi Riau, yang masih digunakan oleh sebagian warga untuk aktivitas sehari-hari. Dendrogram yang dihasilkan dari gabungan 3 primer RAPD-PCR mampu membedakan tiap isolate uji. Intensitas penggunaan air sungai oleh warga dan kuatnya arus mempengaruhi distribusi mikroba indicator sanitasi tersebut dari Tapung hingga Perawang. Sifat resistensi antibiotika masih tergolong rendah (MAR index 0,142), namun eritromisin bukan merupakan antibiotika pilihan untuk diare akibat E.coli disebabkan seluruh isolate resisten terhadap antibiotika ini.


Kata Kunci


Antibiotika; Escherichia coli; RAPD-PCR; Sungai Siak

Teks Lengkap:

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Referensi


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DOI: https://doi.org/10.25077/jsfk.6.2.141-146.2019

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