Penggunaan DNA Mitokondria Sebagai Penanda Sumber Gelatin Sediaan Gummy dengan Teknik Polymerase Chain Reaction dan Sekuensing DNA

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Published: May 29, 2019
DOI: https://doi.org/10.25077/jsfk.6.1.25-31.2019
Keywords:
DNA Mitokondria, Gelatin, Gummy, PCR, Sekuensing DNA.

Main Article Content

Andzar Fikranus Shofa
Program Studi Farmasi, Sekolah Tinggi Ilmu Kesehatan Prima Indonesia
Hariyanti Hariyanti
Universitas Muhammadiyah Prof Dr. HAMKA
Priyo Wahyudi
Badan Pengkajian dan Penerapan Teknologi

Abstract

Chewable lozenges atau gummy merupakan sediaan berbentuk kenyal yang dapat melepaskan zat aktifnya langsung di dalam mulut atau tenggorokan. Bahan yang berpengaruh dalam konsistensi gummy tersebut berasal dari basis. Dalam sediaan gummy, gelatin digunakan sebagai basis yang sebagian besar bersumber dari babi dan sapi. Identifikasi sumber bahan dapat dilakukan dengan teknik PCR dan sekuensing DNA. Penelitian ini bertujuan mengetahui sumber gelatin sediaan gummy impor tanpa logo halal. Isolasi DNA genom daging babi dan sediaan gummy dilakukan dengan GeneJet Kit. Isolat DNA kemudian diamplifikasi menggunakan primer spesifik DNA mitokondria sitokrom b. Selanjutnya, amplikon dianalisis dengan elektroforesis dan dilakukan sekuensing DNA. Hasil elektroforesis amplikon daging babi dan sediaan gummy A menghasilkan pita DNA dengan ukuran 553 bp. Analisis sekuensing DNA menunjukkan homologi dengan Sus scrofa breed long lin. Berdasarkan hasil tersebut dapat disimpulkan gelatin yang digunakan pada sediaan gummy A mengandung gelatin yang berasal dari babi Sus scrofa breed long lin.

Article Details

How to Cite
Shofa, A. F., Hariyanti, H., & Wahyudi, P. (2019). Penggunaan DNA Mitokondria Sebagai Penanda Sumber Gelatin Sediaan Gummy dengan Teknik Polymerase Chain Reaction dan Sekuensing DNA. Jurnal Sains Farmasi & Klinis, 6(1), 25–31. https://doi.org/10.25077/jsfk.6.1.25-31.2019
Issue
Vol. 6 No. 1 (2019): J Sains Farm Klin 6(1), April 2019
Section
Research Articles

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