Isolation and BSLT test of ethyl acetate extract of Shorea ovalis [Kort.] leaves
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Published:
May 5, 2015
Keywords:
Isolation, characterization, ethyl acetate extract of Shorea ovalis (Kort.) leaves, BSLT test.
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Abstract
Isolation and test of Brine Shrimp Lethality Test (BSLT) of ethyl acetate extract of Shorea ovalis [Kort.]) leaves have been done. The isolation method used was column chromatography by Step Gradient Polarity (SGP). The aim of this research was to isolate the metabolite secondary compound and examine the activity test of Brine Shrimp Lethality Test (BSLT) from ethyl acetate extract of Shorea ovalis [Kort.]) leaves. The result showed that pure compound was I¬A which characterized by spectrum of IR, H-NMR, C-NMR, HSQC, HMBC and colour reaction of Liebermann-Burchard test. The characterization of the isolate was phytosterol with estimated molecular formula C27H48O. Result of BSLT test revealed that of ethyl acetat extract of meranti sabut leaves at 100, 10, and 1 ppm had value of LC50= 40.45 ppm with death of larva of Artemia salina equal to 56,6 % and was considered as very toxic.
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Mora, E., Nst, M. R., Susanti, E., & Zasliadi, A. (2015). Isolation and BSLT test of ethyl acetate extract of Shorea ovalis [Kort.] leaves. Jurnal Sains Farmasi & Klinis, 1(2), 184–194. https://doi.org/10.29208/jsfk.2015.1.2.34
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Research Articles
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2. Hakim, E.H., 2002, Oligostilbenoid dari tumbuhan Dipterocarpaceae. Buletin of the Indonesian Socienty of Natural Product Chemistry.2. 1-19.
3. Cronquist, A., 1981, An Integrated System of Classification of Flowering Plants, Columbia, University Press, New York, 316-318.
4. Noviany and Sutopo Hadi, 2009, The Isolation of α- Viniferin, a Trimer Stilbene from Shorea ovalis Blume, Modern Apll.Scie., 3(4); 2009.
5. Sutopo Hadi dan Noviany, 2009, The Isolation of Hopeaphenol, a Tetramer Stilbene from Shorea ovalis Blume, Advances, in Nat. Apll.Scie., 3(1); 107-112.
6. Sarayobudiyono, et all, 2008, Oligostilbenoids from Shorea gibbosa andtheir cytotoxic properties agains P-388 cells. J. Nat Med. 62. 195-198.
7. Harborne, J.B., 1987, Metode Fitokimia Penentuan Cara Modern Menganalisis Tumbuhan. Edisi Ke-2. Terjemahan K. Padmawinata dan I. Soediro, Penerbit ITB, Bandung.
8. Farnsworth, N. R., 1966, Biological and Phytochemical Screening of Plants,J. Pharm Sci. 55, 225-276.
9. Anonim, 2000, Standarisasi Ekstrak, Direktorat Jenderal Pengawasan Obat dan Makanan, Departemen Kesehatan Republik Indonesia. Jakarta.
10. Iskandar, A., dan Bantacut, T., 1990, Kristalisasi,Argo Industri. Press,Bandung
11. Meyer, B.N.R., Ferrigni, J.E., Putnam L,B., Jacobsen. D.E., Nicholas, and Laughin,JL., 1982, Brine Shrimp : A Convenient General Bioassay For Active Plant Constituen. J. Of Medical Plant Medica. 45, 31-34.
12. Harefa, F., 1987, Pembudidayaan Artemia salina untuk Pakar Udang dan Ikan, Penerbit Swadaya, Jakarta.
13. Priyanto, 2009, Toksikologi Mekanisme, Terapi Antidotum, dan Penilaian resiko, Penerbit Lembaga Studi dan Konsultasi Farmakologi (Leskonfi,) Depok.
14. Stahl, E., and Michigan., 1985, Analisis Obat Secara Kromatografi dan Mikroskopi,Terjemahan Padmawinata, K, ITB Press, Bandung.
15. Darwis, D., 2000, Teknik Dasar Laboratorium Dalam Penelitian Senyawa Bahan Alam Hayati, FMIPA, Universitas Andalas, Padang.
16. Sastrohamidjojo, H, 1992, Spektroskopi Infra Merah, Liberty Yogyakarta, Yogyakarta.
17. Silverstein, M.R., 1991, Penyidikan Spektrofotometrik Senyawa Organik. Edisi IV, Terjemahan Hartomo. Erlangga, Jakarta.
18. Mohrig, R.J., Hammond, N.C., Schatz., F.P., and Morrill. C.T. 2003. Techniques in Organik Chemistry. W.H. Freeman Company.
19. Pavia., Lampman., and Kriz. 1996. Introduction to Spectroscophy. Second edition. W. B. Saunder, Philadelphia.
20. Crews, P., Rodriguez, J. and Jaspars, M. 1998. Organic Structure Analysis. University of California, Santa Cruz.