Evaluasi Sitotoksik Alfa Mangostin Pada Kultur Sel Leukosit Manusia Secara In Vitro dan Uji Aktivitas Antioksidan

Article Sidebar

PDF (Bahasa Indonesia)
Published: Feb 8, 2019
DOI: https://doi.org/10.25077/jsfk.5.3.201-206.2018
Keywords:
α-mangostin, sitotoksik, antioksidant, kultur sel leukosit, MTT assay, DPPH assay

Main Article Content

Fatma Sri Wahyuni
Fakultas Farmasi Universitas Andalas
Ikhwan Resmala Sudji
Rizki Amaliyah Amaliyah

Abstract

Ekstrak kulit manggis telah banyak digunakan sebagai obat herbal untuk pengobatan berbagai penyakit dan pemeliharaankesehatan. Senyawa kimia utama dari ekstrak kulit manggis adalah α-mangostin. Bioaktivitas senyawa alfa mangostin telah diuji secara ekstensif pada berbagai organisme in vitro. Namun, informasi tentang efek keamanannya belum diketahui. Penelitian ini bertujuan untuk meninjau keamanan alfamangostin dengan mengevaluasi aktivitas sitotoksik dari alfa mangostin pada kultur sel leukosit normal dan aktivitas antioksidan dari α-mangostin. Evaluasi sitotoksik dilakukan dengan 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay)dan aktivitas antioksidan dilakukan menggunakan1,1-difenil-2-pikrilhidrazil (DPPH assay). Konsentrasi alpha mangostin yang diuji yaitu: 3.125 μg/mL; 6,25 μg/mL; 12,5 μg/mL; 25 μg/mL; 50 μg/mL dan 100 μg/mL. Hasilnya menunjukkan bahwa alfa mangostintidak memiliki efek toksik padakulturselleukosit. Alfamangostin mampu memicu respon proliferasi sel leukosit pada 50 μg/mL dengan viabilitas sel 208,485 ± 21,21% dan pada 100 μg/mL dengan viabilitas sel 361.818 ± 86,37%. Alpha mangostinjuga menunjukkan aktivitas antioksidan yang kuat dengan nilai IC5013,57 μg/mL. Hasil penelitian ini membuktikan bahwa alfa mangostin tidak toksikpada sel leukosit manusia sehingga aman untuk dikonsumsi. Sebagai senyawa antioksidan alfamangostin dapat memicu proliferasi sel leukosit dalam kultur sel leukosit.

Article Details

How to Cite
Wahyuni, F. S., Sudji, I. R., & Amaliyah, R. A. (2019). Evaluasi Sitotoksik Alfa Mangostin Pada Kultur Sel Leukosit Manusia Secara In Vitro dan Uji Aktivitas Antioksidan. Jurnal Sains Farmasi & Klinis, 5(3), 201–206. https://doi.org/10.25077/jsfk.5.3.201-206.2018
Issue
Vol. 5 No. 3 (2018): J Sains Farm Klin 5(3), Desember 2018
Section
Research Articles

The authors retain the copyright and grant the journal the right of first publication simultaneously under the Creative Commons Attribution License. This license allows others to share the work with proper acknowledgment of authorship and initial publication in this journal. Authors are permitted and encouraged to deposit their articles in institutional repositories, on their personal websites, or in other online repositories after the article has been published in JSFK.

Author Biographies

Fatma Sri Wahyuni, Fakultas Farmasi Universitas Andalas

Faculty of Pharmacy, Andalas University

Ikhwan Resmala Sudji

Faculty of Medicine, Andalas University

Rizki Amaliyah Amaliyah

Faculty of Pharmacy, Andalas University

References

Chen LG, Yang LL, Wang CC. Anti-inflammatory activity of mangostins from Garcinia mangostana. Food Chem Toxicol. 2008;46(2):688–93.

Joshua D D, T.Gautschi J, Whitman S, Johnson TA, Gassner NC, Crews P, et al. Discovery of platelet- type 12-human lipoxygenase selective inhibitors by high-throughput screening of structurallay diverse libraries. Bioorg Med Chem. 2007;15(22):1–18.

Cui J, Hu W, Cai Z, Liu Y, Li S, Tao W, et al. New medicinal properties of mangostins: Analgesic activity and pharmacological characterization of active ingredients from the fruit hull of Garcinia mangostana L. Pharmacol Biochem Behav. 2010;95(2):166–72.

Wahyuni FS, Shaari K, Stanslas J, Lajis NH. Cytotoxic xanthones from the stem bark of Garcinia cowa Roxb. J Chem Pharm Res. 2015;7(1):227–36.

Novilla A, Djamhuri DS, Fauziah N, Maesaroh, Balqis, Widowati W. Cytotoxic Activity of Mangosteen (Garcinia mangostana L.) Peel Extract and α-Mangostin toward Leukemia Cell Lines (HL-60 and K-562). J Nat remedies. 2016;16(2):52–9.

Aisha AFA, Abu-Salah KM, Ismail Z, Majid AMSA. In vitro and in vivo anti-colon cancer effects of Garcinia mangostana xanthones extract. BMC Complement Alternative Medication. 2012;12:1.

Yousif M, Mohd N, Mariod AA, Mohan S, Ameen M, Ibrahim S. a -Mangostin from Garcinia mangostana Linn : An updated review of its pharmacological properties. Arab J Chem. 2016;9(3):317–29.

Likhitwitayawuid K, Phadungcharoen T, Krungkrai J. Antimalarial xanthones from Garcinia cowa. Planta Med. 1998;64(April 1994):70–2.

Tjahjani S. Antimalarial activity of Garcinia mangostana L rind and its synergistic effect with artemisinin in vitro. BMC Complement Altern Med. 2017;17(1):131.

Panthong K, Pongcharoen W, Phongpaichit S, Taylor WC. Tetraoxygenated xanthones from the fruits of Garcinia cowa. Phytochemistry. 2006;67(10):999–1004.

Auranwiwat C, Trisuwan K, Saiai A, Pyne SG, Ritthiwigrom T. Antibacterial tetraoxygenated xanthones from the immature fruits of Garcinia cowa. Fitoterapia. 2014;98:179–83.

Ngawhirunpat T, Opanasopi P, Sukma M, Sittisombut C, Kat A, Adachi I. Antioxidant, free radical-scavenging activity and cytotoxicity of different solvent extracts and their phenolic constituents from the fruit hull of mangosteen (Garcinia mangostana). Pharm Biol. 2010;48(1):55–62.

Lin J, Gao Y, Li H, Zhang L, Li X. DNA protective effect of mangosteen xanthones: An in vitro study on possible mechanisms. Adv Pharm Bull. 2014;4(2):147–53.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1–2):55–63.

Kasemwattanaroj P, Moongkarndi P, Pattanapanyasat K, Mangmool S, Rodpai E, Samer J, et al. Immunomodulatory activities of alpha-mangostin on peripheral blood mononuclear cells. Nat Prod Communications. 2013;8(9):1257–60.

Van de Loosdrecht AA, Beelen RHJ, Ossenkoppele GJ, Broekhoven MG, Langenhuijsen MMAC. A tetrazolium-based colorimetric MTT assay to quantitate human monocyte mediated cytotoxicity against leukemic cells from cell lines and patients with acute myeloid leukemia. J Immunol Methods. 1994;174(1–2):311–20.

Knight JA. Review : Free Radicals, Antioxidants, and the Immune System. 2000;30(2):145–58.

Coquette A, Vray B, Vanderpas J. Role of vitamin E in the protection of the resident macrophage membrane against oxidative damage. Arch Int Physiol Biochim. 1986;94:529-534.

Marquez-Valadez B, Maldonado PD, Galvan-Arzate S, Mendez-Questa LA, Perez-De La Cruz V, Pedraza-Chaverri J, Cardenas MEC, Santamaria A. Alpha-mangostin induces changes in glutathione levels associated with glutathione peroxidase activity in rat brain synaptosomes. Journal Nutricional Neuroscience. 2012;15(5):13-19.