Determination of Profenofos Pesticidal Residue in Lettuce (Lactuca sativa L.) by Gas Chromatographic Method
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Published:
May 5, 2015
Keywords:
pesticide residue, profenofos, Lactuca sativa L., gas chromatography
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Abstract
The determination of profenofos pesticidal residue in the lettuce (Lactuca sativa L.) by using gas chromatography using flame photometric detector (FPD) had been investigated. The lettuce was collected from Padang Luar area, Agam distric, West Sumatera. Sample for determination of profenofos residue divided into three groups: unwashed (A), washed with water (B), and washed with detergent (C). Maceration with sonication was used for the extraction using ethylacetateas a solvent. The results showed that profenofos pesticide residue in sample A, B and C were 0.204, 0.080 and 0.061 ppm, respectively. These profenofos pesticidal residue are over than the Maximum Residue Limits (MRL) that established by The Japan Food Chemical Research Foundation (0.05 ppm) even though World Health Organization (WHO) has not established Maximum Residue Limits (MRL) profenofos on lettuce. Based on the statistical analysis one-way method (Anova) using SPSS 20.0 showed that there was a significant concentrations difference between lettuce A from lettuce B and lettuce C with p < 0.05.
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Alen, Y., Zulhidayati, Z., & Suharti, N. (2015). Determination of Profenofos Pesticidal Residue in Lettuce (Lactuca sativa L.) by Gas Chromatographic Method. Jurnal Sains Farmasi & Klinis, 1(2), 140–149. https://doi.org/10.29208/jsfk.2015.1.2.30
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Research Articles
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References
1. Rubatzky, V., & Yamaguchi, M. (1998). Sayuran Dunia 2: Prinsip, Produksi dan Gizi, Edisi 2. Penerjemah: Catur Herison. Bandung: Institut Teknologi Bandung.
2. Chen, C., Qian, Y., Chen, Q., Tao, C., Li, C. & Li, Y. (2011). Evaluation of Pesticide Residues in Fruits and Vegetables from Xiamen. Food Control, 22, 1114-1120.
3. Munarso, S.J., Miskiyah & Broto, W. (2006). Studi Kandungan Residu Pestisida pada Kubis, Tomat, dan Wartel di Malang dan Cianjur. Buletin Teknologi Pascapanen Pertanian, 2.
4. Indrayani, N. (2006). Bioremediasi Lahan Tercemar Profenofos Secara Ex-situ dengan Cara Pengomposan. (Tesis). Bogor: Institut Pertanian Bogor.
5. Alen, Y., Habazar, T., Syarif, Z. & Tajib, G. (2013). Rancang Bangun Model Pengembangan Agribisnis Sayuran Sehat melalui Sumber Daya Lokal untuk Peningkatan Daya Saing dan Pendapatan Petani di Kabupaten Agam (Laporan Penelitian Program Penelitian Unggulan Strategis Nasional No: 02/UN.16/PL-USN/2013). Padang: Universitas Andalas.
6. Quijano, R. & Sarojeni, V.R. (1999). Pestisida Berbahaya Bagi Kesehatan. Solo: Yayasan Duta Awam.
7. Alavanja, M.C.R., Hoppin, J.A. & Kamel, F. (2009). Health Effects of Chronic Pesticide Exposure. Cancer and Neurotoxicity Annual Review of Public Health, 25, 155-197.
8. Gandjar, I.G. & Abdul, R. (2007). Kimia Farmasi Analisis. Yogyakarta: Pustaka Pelajar.
9. Susilowati, A.L., Primaharinastiti, R. & Soerjono, J. (2012). Optimasi Metode Ekstraksi Untuk Analisis Triadimefon Pada Kubis Secara Kromatografi Gas-Spektrometri Massa (Laporan Penelitian). Surabaya: Lembaga Penelitian Universitas Airlangga.
10. Yulnefi. (2001). Pemeriksaan Residu Pestisida Organofosfat Dalam Tanaman Kubis (Brassica olerecea L.) dengan Metoda Kromatografi Gas. (Skripsi). Padang: Universitas Andalas.
11. Sweetman, S.C. (2009). Martindale The Complete Drug Reference (36th ed). London: Pharmaceutical Press.
12. Wonnacott, R.J. & Wonnacott, T.H. (1989). Pengantar Statistika (Edisi 4). Penerjemah: Bambang Sumantri. Jakarta: Erlangga.
13. Harmita. (2004). Petunjuk Pelaksanaan Validasi, Metode, dan Cara Perhitungannya. Majalah Ilmu Kefarmasian, I, 3, 117-135.
14. Association of Official Analytical Chemist. (2012). Guidelines for Single Laboratory Validation of Chemical Methods for Dietary Supplements and Botanicals, Diakses 11 September 2014 dari www.aoac.org/official_methods/slv_guidelines.pdf.
15. The Japan Food Chemical Research Foundation. (2006). Pesticides Profenofos. Diakses 20 Mei 2014 dari https://www.m5.ws001.squarestart.ne.jp/ foundation/fooddtl.php.
16. Anonim. (2008). Batas Maksimum Residu Pestisida Pada Hasil Pertanian. Jakarta: Badan Standarisasi Nasional.
17. Anonim. (2007). Pesticide Residues in Food (Report of the Joint Meeting of the FAO Panel of Experts on Pesticide Residues in Food and the Environment and the WHO Core Assessment Group, 18-27 September 2007). Geneva: Food and Agriculture Organizations & World Health Organizations.
18. Lukitaningsih, E., Sudarmanto, B.S.A. & Noegrohati, S. (2002). Uji Efektifitas Daya Bersih Beberapa Sediaan Surfaktan Terhadap Residu Pestisida Pada Buah Apel Segar. Majalah Farmasi Indonesia, 13, 4, 200-206.
19. Miskiyah & Munarso, S.J. (2009). Kontaminasi Residu Pestisida pada Cabai Merah, Selada, dan Bawang Merah. (Studi Kasus di Bandungan dan Brebes Jawa Tengah serta Cianjur Jawa Barat. J. Hort, 19, 1,101-111.
20. Amvrazi, E.G. (2011). Pesticides - The Impacts of Pesticide Exposure. M. Stoytcheva (Ed). Fate of Pesticide Residues on Raw Agricultural Crops after Postharvest Storage and Food Processing to Edible Portions, Pesticides-Formulations, Effects, Fate. 576-588. Rijeka: Intech.
21. Atmawidjaja, S., Tjahjono, D.H. & Rudiyanto. (2004). Pengaruh Perlakuan Terhadap Kadar Residu Pestisida Metidation Pada Tomat. Acta Pharmaceutica Indonesia, 29, 2, 72-82.
22. Gonzales, R.M., Rial, O.R., Cancho, G.B. & Simal, G.J. (2007). Occurrence of Fungicide and Insecticide Residues in Trades Samples of Leafy Vegetables. Food Chem, 107, 3, 1342-1347.
23. De Vreede, J.A.F., Brouwer, D.H., Stevenson, H. & Hemmen, J.J.V. (1998). Exposure and Risk Estimation for Pesticides in High-volume Spraying. British Occupational Hygiene Society, 42, 3, 151-157.
24. Barile, F.A. (2003). Clinical Toxicology: Principles and Mechanisms. London: CRC Press.
25. Romeo & Rengam. (1999). Awas Pestisida Berbahaya Bagi Kesehatan. Solo: Yayasan Duta Awam.
26. Adriyani, R. (2006). Usaha Pengendalian Pencemaran Lingkungan Akibat Penggunaan Pestisida Pertanian. Jurnal Kesehatan Lingkungan, 3, 1, 95-106.
2. Chen, C., Qian, Y., Chen, Q., Tao, C., Li, C. & Li, Y. (2011). Evaluation of Pesticide Residues in Fruits and Vegetables from Xiamen. Food Control, 22, 1114-1120.
3. Munarso, S.J., Miskiyah & Broto, W. (2006). Studi Kandungan Residu Pestisida pada Kubis, Tomat, dan Wartel di Malang dan Cianjur. Buletin Teknologi Pascapanen Pertanian, 2.
4. Indrayani, N. (2006). Bioremediasi Lahan Tercemar Profenofos Secara Ex-situ dengan Cara Pengomposan. (Tesis). Bogor: Institut Pertanian Bogor.
5. Alen, Y., Habazar, T., Syarif, Z. & Tajib, G. (2013). Rancang Bangun Model Pengembangan Agribisnis Sayuran Sehat melalui Sumber Daya Lokal untuk Peningkatan Daya Saing dan Pendapatan Petani di Kabupaten Agam (Laporan Penelitian Program Penelitian Unggulan Strategis Nasional No: 02/UN.16/PL-USN/2013). Padang: Universitas Andalas.
6. Quijano, R. & Sarojeni, V.R. (1999). Pestisida Berbahaya Bagi Kesehatan. Solo: Yayasan Duta Awam.
7. Alavanja, M.C.R., Hoppin, J.A. & Kamel, F. (2009). Health Effects of Chronic Pesticide Exposure. Cancer and Neurotoxicity Annual Review of Public Health, 25, 155-197.
8. Gandjar, I.G. & Abdul, R. (2007). Kimia Farmasi Analisis. Yogyakarta: Pustaka Pelajar.
9. Susilowati, A.L., Primaharinastiti, R. & Soerjono, J. (2012). Optimasi Metode Ekstraksi Untuk Analisis Triadimefon Pada Kubis Secara Kromatografi Gas-Spektrometri Massa (Laporan Penelitian). Surabaya: Lembaga Penelitian Universitas Airlangga.
10. Yulnefi. (2001). Pemeriksaan Residu Pestisida Organofosfat Dalam Tanaman Kubis (Brassica olerecea L.) dengan Metoda Kromatografi Gas. (Skripsi). Padang: Universitas Andalas.
11. Sweetman, S.C. (2009). Martindale The Complete Drug Reference (36th ed). London: Pharmaceutical Press.
12. Wonnacott, R.J. & Wonnacott, T.H. (1989). Pengantar Statistika (Edisi 4). Penerjemah: Bambang Sumantri. Jakarta: Erlangga.
13. Harmita. (2004). Petunjuk Pelaksanaan Validasi, Metode, dan Cara Perhitungannya. Majalah Ilmu Kefarmasian, I, 3, 117-135.
14. Association of Official Analytical Chemist. (2012). Guidelines for Single Laboratory Validation of Chemical Methods for Dietary Supplements and Botanicals, Diakses 11 September 2014 dari www.aoac.org/official_methods/slv_guidelines.pdf.
15. The Japan Food Chemical Research Foundation. (2006). Pesticides Profenofos. Diakses 20 Mei 2014 dari https://www.m5.ws001.squarestart.ne.jp/ foundation/fooddtl.php.
16. Anonim. (2008). Batas Maksimum Residu Pestisida Pada Hasil Pertanian. Jakarta: Badan Standarisasi Nasional.
17. Anonim. (2007). Pesticide Residues in Food (Report of the Joint Meeting of the FAO Panel of Experts on Pesticide Residues in Food and the Environment and the WHO Core Assessment Group, 18-27 September 2007). Geneva: Food and Agriculture Organizations & World Health Organizations.
18. Lukitaningsih, E., Sudarmanto, B.S.A. & Noegrohati, S. (2002). Uji Efektifitas Daya Bersih Beberapa Sediaan Surfaktan Terhadap Residu Pestisida Pada Buah Apel Segar. Majalah Farmasi Indonesia, 13, 4, 200-206.
19. Miskiyah & Munarso, S.J. (2009). Kontaminasi Residu Pestisida pada Cabai Merah, Selada, dan Bawang Merah. (Studi Kasus di Bandungan dan Brebes Jawa Tengah serta Cianjur Jawa Barat. J. Hort, 19, 1,101-111.
20. Amvrazi, E.G. (2011). Pesticides - The Impacts of Pesticide Exposure. M. Stoytcheva (Ed). Fate of Pesticide Residues on Raw Agricultural Crops after Postharvest Storage and Food Processing to Edible Portions, Pesticides-Formulations, Effects, Fate. 576-588. Rijeka: Intech.
21. Atmawidjaja, S., Tjahjono, D.H. & Rudiyanto. (2004). Pengaruh Perlakuan Terhadap Kadar Residu Pestisida Metidation Pada Tomat. Acta Pharmaceutica Indonesia, 29, 2, 72-82.
22. Gonzales, R.M., Rial, O.R., Cancho, G.B. & Simal, G.J. (2007). Occurrence of Fungicide and Insecticide Residues in Trades Samples of Leafy Vegetables. Food Chem, 107, 3, 1342-1347.
23. De Vreede, J.A.F., Brouwer, D.H., Stevenson, H. & Hemmen, J.J.V. (1998). Exposure and Risk Estimation for Pesticides in High-volume Spraying. British Occupational Hygiene Society, 42, 3, 151-157.
24. Barile, F.A. (2003). Clinical Toxicology: Principles and Mechanisms. London: CRC Press.
25. Romeo & Rengam. (1999). Awas Pestisida Berbahaya Bagi Kesehatan. Solo: Yayasan Duta Awam.
26. Adriyani, R. (2006). Usaha Pengendalian Pencemaran Lingkungan Akibat Penggunaan Pestisida Pertanian. Jurnal Kesehatan Lingkungan, 3, 1, 95-106.