Development and Validation of Loperamide Hydrochloride Tablet Analysis Method with Absorbance and Area under Curve Methods Spectrophotometrically

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Published: Oct 11, 2018
DOI: https://doi.org/10.25077/jsfk.5.2.94-101.2018
Keywords:
Loperamide hydrochloride, absorbance method, area under the curve method, spectrophotometry

Main Article Content

Harrizul Rivai
Faculty of Pharmacy, Andalas University, Limau Manih Campus, Padang 25163
https://orcid.org/0000-0003-4569-8425
Renny Puspita
School of Pharmaceutical Science Kurao, Siteba, Padang 25138
Sesry Misfadhila
School of Pharmaceutical Science Kurao, Siteba, Padang 25138

Abstract

Development and validation of loperamide hydrochloride tablet method of analysis with absorbance method and area under the curve method have been done. This study used methanol and hydrochloric acid 0.1 N (9: 1) as the best solvent with maximum absorption at wavelength 259.00 nm. Linearity of loperamide hydrochloride was obtained in the concentration range 200-600 ppm with correlation coefficient value with absorbance method and the areas under the curve method were 0.9998 and 0.9865, respectively. The results showed that the levels of loperamide hydrochloride in generic tablets obtained by absorbance method and the area under the curve method were 105.71% and 96.20%, respectively. The results of determination of loperamide levels in tablets of trademark obtained by absorbance method and the area under the curve method were 102.85% and 98.57%, respectively. The level of both samples met the requirements of Pharmacopoeia Indonesia edition V that is 90% -110%.

Article Details

How to Cite
Rivai, H., Puspita, R., & Misfadhila, S. (2018). Development and Validation of Loperamide Hydrochloride Tablet Analysis Method with Absorbance and Area under Curve Methods Spectrophotometrically. Jurnal Sains Farmasi & Klinis, 5(2), 94–101. https://doi.org/10.25077/jsfk.5.2.94-101.2018
Issue
Vol. 5 No. 2 (2018): J Sains Farm Klin, Vol 5 No 2, August 2018
Section
Research Articles

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Author Biography

Harrizul Rivai, Faculty of Pharmacy, Andalas University, Limau Manih Campus, Padang 25163

Fakultas Farmasi Universitas Andalas

Profesor

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