Development and Validation of Loperamide Hydrochloride Tablet Analysis Method with Absorbance and Area under Curve Methods Spectrophotometrically

Harrizul Rivai, Renny Puspita, Sesry Misfadhila

Abstrak


Development and validation of loperamide hydrochloride tablet method of analysis with absorbance method and area under the curve method have been done. This study used methanol and hydrochloric acid 0.1 N (9: 1) as the best solvent with maximum absorption at wavelength 259.00 nm. Linearity of loperamide hydrochloride was obtained in the concentration range 200-600 ppm with correlation coefficient value with absorbance method and the areas under the curve method were 0.9998 and 0.9865, respectively. The results showed that the levels of loperamide hydrochloride in generic tablets obtained by absorbance method and the area under the curve method were 105.71% and 96.20%, respectively. The results of determination of loperamide levels in tablets of trademark obtained by absorbance method and the area under the curve method were 102.85% and 98.57%, respectively. The level of both samples met the requirements of Pharmacopoeia Indonesia edition V that is 90% -110%.

Kata Kunci


Loperamide hydrochloride; absorbance method; area under the curve method; spectrophotometry

Teks Lengkap:

PDF (English)

Referensi


Trevor AJ, Katzung BG, Kruidering-Hall M; Katzung & Trevor’s Pharmacology Examination & Board Review. 11th Edition, Lange McGraw-Hill Education, New York, 2015; 158

Kementerian Kesehatan RI; Farmakope Indonesia, Edisi V, Kementerian Kesehatan RI, Jakarta, 2014; 775-777.

Sujatha T, Balmuralikrishna K, & Raju RR. A validated RP-HPLC method for the estimation of loperamide hydrochloride in tablet dosage forms. International Journal of Chem Tech Research. 2014;6(2):1097-1102.

Savić IM, Nikolić GS, Savić IM & Marinković VD. Quantitative analysis of Loperamide hydrochloride in the presence its acid degradation products. Hemijska industrija. 2009;63(1):39-46.

Ruddy DA & Sherma J. Determination of the active ingredient loperamide hydrochloride in pharmaceutical caplets by high performance thin layer chromatography with ultraviolet absorption densitometry of fluorescence quenched zones. Acta poloniae pharmaceutica. 2002;59(1):15-18.

Faridbod F, Mizani F, Ganjali MR, & Norouzi P. Potentiometric determination of loperamide hydrochloride by loperamide PVC membrane and nano-composite electrodes. International Journal of Electrochemical Science. 2012;7(8):7643-7654.

Singh L, & Nanda S. Validated Spectrophotometric methods for estimation of Loperamide Hydrochloride from tablet dosage form. Asian Journal of Pharmaceutical and Clinical Research. 2010;3(2):121-122.

El Sherif ZA, Mohamed AO, Walash MI, & Tarras FM. Spectrophotometric determination of loperamide hydrochloride by acid-dye and charge-transfer complexation methods in the presence of its degradation products. Journal of pharmaceutical and biomedical analysis. 2000;22(1):13-23.

Rivai H, Hasanah R, Azizah Z. Development and Validation of Omeprazole Analysis Methods in Capsules with Absorbance Methods and Areas under Curves Methods with UV-Vis Spectrophotometry. International Journal of Pharmaceutical Sciences and Medicine (IJPSM). 2018; 3(3): 21-32.

Asra R, Rivai H, Riani VL. Pengembangan dan Validasi Metode Analisis Tablet Furosemid dengan Metode Absorbansi dan Luas Daerah di Bawah Kurva secara Spektrofotometri Ultraviolet. Jurnal Farmasi Higea. 2016; 8(2): 110-121.

Chandra B, Rivai H, Apriansyah E. Pengembangan dan Validasi Metode Analisis Propranolol Hidroklorida Tablet dengan Metode Absorbansi dan Luas Daerah di Bawah Kurva secara Spektrofotometri Ultraviolet. Jurnal Farmasi Higea. 2017; 9(1): 20-29.

Chandra B, Rivai H, Marianis M. Pengembangan dan Validasi Metode Analisis Ranitidin Hidroklorida Tablet dengan Metode Absorbansi dan Luas Daerah di Bawah Kurva secara Spektrofotometri Ultraviolet. Jurnal Farmasi Higea. 2016; 8(2): 96-109.

Rivai H, Astuty W, Asra R. Pengembangan dan Validasi Metode Analisis Betametason dalam Tablet dengan Metode Absorbansi dan Luas Daerah di Bawah Kurva Secara Spektrofotometri Ultraviolet. Jurnal Sains dan Teknologi Farmasi. 2017; 19(Supl1): s52-s57.

Rivai H, Larasaky M, Azizah Z. Pengembangan dan Validasi Metode Analisis Klorfeniramin Maleat Dalam Tablet dengan Metode Absorbansi dan Luas Daerah di Bawah Kurva Secara Spektrofotometri Ultraviolet. Jurnal Sains dan Teknologi Farmasi. 2017; 19(Supl1): s58-s63.

Rivai H, Pratama N, Asra R. Development and Validation of Bisacodyl Analysis Method in Tablet with Absorbance Method and Area under Curves Method in Ultraviolet Spectrophotometry. International Journal of Pharmaceutical Sciences and Medicine. 2017; 2(12): 1-8.

Rivai H, Nofera NS, Azizah Z. Development and Validation of Dimenhydrinate Analysis Method in Tablet with Absorbance Method and Method of Area under Curve with Ultraviolet Spectrophotometry. Scholars Academic Journal of Pharmacy. 2018;7(3): 155-163.

Rohman A. Validasi dan Penjaminan Mutu Metode Analisis Kimia. Yogyakarta: Gajah Mada University Press, 2016; 87-110.

Jones DS. Statistik farmasi. Penerjemah: HU Ramadanianti, & H Rivai. Jakarta: Penerbit Buku Kedokteran EGC, 2010




DOI: https://doi.org/10.25077/jsfk.5.2.94-101.2018

Article Metrics

Abstract view : 484 times
PDF (English) view/download : 1077 times



Jurnal Sains Farmasi & Klinis (J Sains Farm Klin) | p-ISSN: 2407-7062 | e-ISSN: 2442-5435

Diterbitkan oleh Fakultas Farmasi Universitas Andalas bekerjasama dengan Ikatan Apoteker Indonesia - Daerah Sumatera Barat 

      

 JSFK is licensed under Creative Commons Attribution-ShareAlike 4.0 International License.