Characterization of crude drugs and henna leaves extract (Lawsonia inermis Linn) and determination of total phenolic content and antioxidant activity test

Main Article Content

Elidahanum Husni
Netty Suharti
Arlyn Pasella Tri Atma

Abstract

Lawsonia inermis Linn is the plants into the Lythraceae family. This plant has proven to be able to treat a variety of diseases. This research were aimed to identify the characterization of ethanol extracts and simplicia of  henna leaves, determine the total phenolic content with Folin-Ciocalteau method and antioxidant activity with Ferric Reducing Antioxidant Power (FRAP). The extraction was performed using 70 % ethanol. Characterization of simplicia was a powder, greenish brown color, bitter tasted and a distinctive odor. The loss of drying simplisia was 4.92 % ± 0.16, total ash was 4.61 % ± 0.02, acid insoluble ash was 4.43 % ± 0.14, water soluble concentration was 3.30 % ± 0.06, and ethanol soluble concentration was 25.46 % ± 0.18. Characterization of ethanol extracts was viscous, brown in color, typical odor and bitter tasted. The water content of ethanol extracts was 9.98 % ± 2.00, total ash content was 2.63 % ± 0.91, and non acid soluble ash content was 0.36 % ± 0.11. The results of the determination of the total phenolics of ethanol extracts was 16.02 g/100 g. And antioxidant activity of ethanol extract was 2.85 mmol Fe (II)/100 g

Article Details

How to Cite
Husni, E., Suharti, N., & Atma, A. P. T. (2018). Characterization of crude drugs and henna leaves extract (Lawsonia inermis Linn) and determination of total phenolic content and antioxidant activity test. Jurnal Sains Farmasi & Klinis, 5(1), 12–16. https://doi.org/10.25077/jsfk.5.1.12-16.2018
Section
Research Articles

References

Hasiani VV, Ahmad I, Rijai L. Isolasi jamur endofit dan produksi metabolit sekunder antioksidan dari daun pacar (Lawsonia inermis L.) Jurnal Sains dan Kesehatan. 2015;1(4):146-153.

Marc EB, Nelly A, Annick DD, Frederic D. Plants used as remedies antirheumatic and antineuralgic in the traditional medicine of Lebanon. J Ethnopharmacol. 2008;34:120-315.

Gbolade AA. Inventory of antidiabetic plants in selected districts of Lagos State, Nigeria. J Ethnopharmacol. 2009;9:121-135.

Wiem A, Smail A, Wissem M, Faleiro M, Miguel M. Antioxidant, antiinflammatory and anti-acetylcholinesterase activities of leaf, flower and seed aqueous extracts of Lawsonia inermis from Tunisia. Int. J. Pharm. Pharm. Sci. 2014;6(5):975-1491.

Hosein HKM, Zinab D. Phenolic compound and antioxidant activity of henna leaves extracts (Lawsonia inermis). World Journal of Dairy & Food Sciences. 2007;2(1):38-41.

Departemen Kesehatan Republik Indonesia. Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: Departemen Kesehatan Republik Indonesia; 2000.

Prakash A, Rigelhof F, Miller E. Antioxidant activity. Medallion Laboratories: Analytical Progress. 2001;19(2):1 – 4.

Mandal S, Yadav S, Nema RK. Antioxidant: a review. Journal of Chemical and Pharmaceutical Research. 2009;1(1):102-104.

Gordon MH. The mechanism of antioxidants actions in vitro. Di dalam : Hudson BJF, editor. Food Antioxidant. Elsevier Applied Science. 1990:1-18.

Fluck H, Jaspersen SR. Medicinal Plants And Their Uses. London: W. Foulsham & Co. Ltd; 1976.

Raskin I, Ripoll C. Can an apple a day keep the doctor away?. Current Pharmaceutical Design. 2004;10:1-9.

Departemen Kesehatan Republik Indonesia. Suplemen II Farmakope Herbal Indonesia. Jakarta: Depkes RI; 2010.

Departemen Kesehatan RI. Farmakope Indonesia Edisi IV. Jakarta: Departemen Kesehatan RI; 1995.

Andayani R, Maimunah, Lisawati Y. Penentuan aktivitas antioksidan, kadar fenolat dan likopen pada buah tomat (Solanum lycopersicum L.). Jurnal Sains dan Teknologi Farmasi. 2008;13:31-37.

Agoes, G. Teknologi Bahan Alam (edisi revisi). Bandung: Penerbit ITB; 2007.

Waterhouse AL. Folin ciocalteau micro method for total phenol in wine. American Journal of Enology and Viticulture. 1999;28:1-3.

Susantika M. Penentuan kadar fenolat total dan uji ativitas antioksidan dari ekstrak etanol kulit batang asam kandis. [Skripsi]. Padang: Fakultas Farmasi Universitas Andalas; 2016.

Badan POM RI. Acuan Sediaan Herbal Vol. 7 Edisi I. Direktorat Obat Asli Indonesia; 2012.

Das PK, Mondal AK. Studies on traditional ‘mehendi’ used as herbal colour with special references to its antimicrobial activity and pigment profile by TLC. International Journal of Science and Nature. 2012;3(4):799-804.

Kementerian Kesehatan RI. Farmakope Herbal Indonesia Suplemen 3 Edisi I. Jakarta: Dirjen Pelayanan Farmasi dan Alat Kesehatan, Kemenkes RI; 2013