Interaction of sambiloto extract (Andrographis paniculata (Burm.F.)Nees) with glibenclamide towards CYP3A4 gene expression in HEPG2 cell line
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ABSTRACT: Diabetes mellitus is one of the chronic metabolic diseases characterized by hyperglycemia. Various therapies are done to overcome hyperglycemia, either with oral antidiabetic drugs or plants that have antidiabetic properties. Sambiloto (Andrographis paniculata (Burm.F.)Ness) is one of antidiabetic plants that is widely used by Indonesian people. However, in many cases, the combination of herbs with synthesis drugs causes interaction when used at the same time. This study was aimed to obtain interaction data of the sambiloto extract with glibenclamide towards CYP3A4 gene expression in HepG2 cell line. The results of this study showed a decreased in the expression of CYP3A4 gene in a single sample test as well as a combination sample at concentrations of 50 and 100 μg/mL for green chiretta extract, glibenclamide and its combination decreased CYP3A4 gene expression respectively 0,82, 0,70; 0,89, 0,53; 0,84, 0,72. It can be concluded there is a decreased in the expression of CYP3A4 gene along with the increase of sample concentration.
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