Determination of Total Phenolic Content and Antioxidant Activity of Epigallocatechin Gallate Rich Fraction from Tea Leaves (Camellia sinensis L.)
Main Article Content
Abstract
The tea plant, Camellia sinensis L., is a well-known natural product with significant health advantages. The main catechin found in green tea leaves is epigallocatechin gallate (EGCG), which has potent antioxidant properties. EGCG is a common active ingredient in products from the medical and cosmetics industries. This study examines the antioxidant properties and total phenolic content (TPC) of an EGCG-rich fraction (ERF) that is extracted from green tea leaves. TPC was quantified using the Folin-Ciocalteu method, with absorbance measured via UV-VIS spectrophotometry at 760.5 nm. Antioxidant activity was assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, with absorbance recorded at 516 nm. The findings indicated that the TPC of ERF was 437.18 mg GAE/g ± 5.54, while the IC50 value for antioxidant activity was 4.47 mg/L, This value was lower than that of the positive control, Vitamin C, which exhibited an IC₅₀ of 5.07 mg/L. These results suggest that ERF could be developed into a natural ingredient for cosmetics and dietary supplements.
Article Details
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
The authors retain the copyright and grant the journal the right of first publication simultaneously under the Creative Commons Attribution License. This license allows others to share the work with proper acknowledgment of authorship and initial publication in this journal. Authors are permitted and encouraged to deposit their articles in institutional repositories, on their personal websites, or in other online repositories after the article has been published in JSFK.
References
[1]. A. N. Azizah, I. Ichwanuddin, and N. Marfu'ah, "Antibacterial Activity of Green Tea Ethanol Extract (Camellia sinensis) Against the Growth of Staphylococcus epidermidis," Pharm. J. Islam. Pharm., vol. 4, no. 2, p. 15, 2020.
[2]. S. Pradhan and R. C. Dubey, “Beneficial properties of green tea,” Antioxid. Prop. Heal. Benefits Green Tea, pp. 27–56, 2021.
[3]. H. R. Rabbani, D. A. Purwanto, and I. Isnaeni, “Effect of Guava Powder Addition on Epigallocatechin Gallate (EGCG) Content of Green Tea and Its Antioxidant Activity,” J. Farm. and Indones Pharmaceutical Sciences., vol. 6, no. 2, p. 85, 2020.
[4]. W. Koch, W. Kukuła-Koch, M. Czop, P. Helon, and E. Gumbarewicz, “The role of extracting solvents in the recovery of polyphenols from green tea and its antiradical activity supported by principal component analysis,” Molecules, vol. 25, no. 9, pp. 1–14, 2020.
[5]. Rumyati, N. Idiawati, and L. Destiarti, "Test of Antioxidant Activity, Total Phenol and Toxicity of Leaves and Stems of Lakum (Cayratia trifolia (L.) Domin," J. Kim. Khatulistiwa, vol. 3, no. 3, pp. 30–35, 2014.
[6]. L. M. M. Candra, Y. Andayani, and D. G. Wirasisya, "Effect of Extraction Method on Total Phenolic and Total Flavonoid Content in Chickpea Ethanol Extract (Phaseolus vulgaris L.)," J. Pijar Mipa, vol. 16, no. 3, pp. 397–405, 2021.
[7]. J. Levita, S. A. Salim, N. M. Saptarini, and F. A. Saputri, "Article Review: Advantages and Limitations of Folinciocalteu Reagents in Determining Total Phenol Levels in Plants," Pharmaceuticals, vol. 18, no. 1, pp. 46–57, 2020.
[8]. N. Suryani Djamain, V. Anggia, and N. Fachrunisa, "Antibacterial Activity Test Of Angelica Leaf Ethanol Extract Gel (Pterocarpus Indicus Willd.) Against Staphylococcus aureus Bacteria," J. Insa. Farm. Indones., vol. 3, no. 1, pp. 124–131, 2020.
[9]. D. Septia Ningsih, H. Henri, O. Roanisca, and R. Gus Mahardika, “Skrining Fitokimia dan Penetapan Kandungan Total Fenolik Ekstrak Daun Tumbuhan Sapu-Sapu (Baeckea frutescens L.),” Biotropika J. Trop. Biol., vol. 8, no. 3, pp. 178–185, 2020.
[10]. Sani, N. F., Wirya, S., & Hermayanti, C. (2016). Formula and Evaluation of Antiacne Spray Gel From Ethyl Acetate Fraction Of Shoot Green Tea Leaf (Camelia sinensis [L.] Kuntze).
[11]. D. Rohdiana and Shabri, "Individual analysis of green tea catechins extracted and column chromatographic fractionation," J. Researcher. Tea and Quinine, Vol. 15, no. September 2007, pp. 81–88, 2012.
[12]. C. Chatimah, Sugijanto, and A. T. Poernomo, "Validation of TLC-Densitometry Method on Determination of Levels of (-)-Epigallocatechin Gallate (EGCG) in Green Tea," Berk. Ilm. Kim. Farm., vol. 7, no. 2, pp. 55–63, 2020.
[13]. A. W. Pratama, R. L. Sri, G. Abdul, and R. Yunita, "Phytochemical Screening, Total Phenols, and Antioxidant Activity of Methanol Extract from Great Banana Fruit Comb Stems," J. Food and Nutrition, vol. 12, no. 2, pp. 14–21, 2022.
[14]. A. Courtney, “Formularies,” Pocket Handb. Nonhum. Primate Clin. Med., pp. 213–218, 2012.
[15]. R. Amalia Rachmawati, N. W. Wisaniyasa, and I. K. Suter, "Effect Of Solvent Type On Antioxidant Activity Of Meniran Extract (Phyllanthus niruri L.)," A. Science and Technology. Food, vol. 9, no. 4, p. 458, 2020.
[16]. R. Supriningrum, H. Nurhasnawati, and S. Faisah, "Determination Of Total Phenolic Content Of Serunai Leaf Ethanol Extract (Chromolaena odorata L.) By Uv-Vis Spectrophotometry Method," Al Ulum J. Science and Technology., vol. 5, no. 2, p. 54, 2020.
[17]. O. Anngela, A. Muadifah, and D. P. Nugraha, "Validation of Borax Level Determination Method in Puli Crackers Using UV-Vis Spectrophotometer," J. Science and Health., vol. 3, no. 4, pp. 375–381, 2021.