Investigating the Impact of Surfactant and Cosolvent on the Polyphenolic Content in Arumanis Mango Leaf Extract (Mangifera indica L.)
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Abstract
The leaf of Mangifera indica L. contains flavonoids and mangiferin which showed positive effects on wound healing of diabetic ulcer. The used of suitable surfactant and co-solvent is required to ensure the high solubility of the active pharmaceutical ingredient (API), thereby optimizing the efficacy. This study aims to determine the ideal surfactant and cosolvent for a thermosensitive hydrogel formula of Mangifera indica leaf ethanol extract. The leaf was fextracted by maceration using 70% ethanol then determined for moisture content. The extract was prepared for solubility tests of flavonoid and mangiferin on surfactants (Kolliphor® EL, Tween® 20, Tween® 80, Miranol® C2M) and co-solvents (Glycerin, PEG 400, and transcutol). Methods for total flavonoids content were Spectrophotometry UV-Vis and mangiferin with RP-HPLC method. The moisture content in the extract is 8.590 ± 0.754%. The surfactant demonstrating the highest capability in flavonoid dissolution is Tween 20 (1863.750 ± 0.838 µgQE/g extract), followed by Tween 80, Kolliphor EL, and Miranol C2M. In the context of co-solvents, PEG 400 (1309.583 ± 0.292 µgQE/g extract) show superior flavonoid dissolution capability, with glycerin and Transcutol following in descending order. Tween 20 and Tween 80 exhibite comparable efficacy in mangiferin dissolution, followed by Miranol C2M and Kolliphor EL. Among co-solvents, Transcutol demonstrate the highest aptitude for mangiferin dissolution, succeeded by PEG 400 and glycerin. This study indicates Tween 20 is a preferable surfactant and PEG 400 was considered as co-solvent to be further used in a thermosensitive gel formula for diabetic ulcer.
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References
Kintoko, K., Karimatulhajj, H., Elfasyari, T. Y., Ihsan, E. A., Putra, T. A., Hariadi, P., Ariani, C., and Nurkhasanah, N., 2017, Effect of Diabetes Condition on Topical Treatment of Binahong Leaf Fraction in Wound Healing Process, Maj. Obat Tradis.,.
Safitri, S., Miyarso, C., and Fitriyati, L., 2022, Uji Anti Luka Bakar Kombinasi Ekstrak Etanol Daun Mangga Arumanis (Mangifera indica L.) Dan Daun Salam (Syzygium polianthum (Wight) Walp.) Terhadap penyembuhan luka bakar derajat II a tikus putih jantan galur wistar, J. Farm. Klin. dan Sains, 2 (2), .
Dumville, J. C., Lipsky, B. A., Hoey, C., Cruciani, M., Fiscon, M., and Xia, J., 2017, Topical antimicrobial agents for treating foot ulcers in people with diabetes, Cochrane Database Syst. Rev., 2017 (6), .
Soliman, K. A., Ullah, K., Shah, A., Jones, D. S., and Singh, T. R. R., 2019, Poloxamer-based in situ gelling thermoresponsive systems for ocular drug delivery applications, Drug Discov. Today, 24 (8), .
Afrinanda, R., 2019, Formulasi Dan Uji Efektivitas Gel Ekstrak Etanol 70% Daun Mangga Arumanis (Mangifera Indica L) Sebagai Penyembuh Luka Diabetes Pada Tikus Jantan Galur Wistar Yang Diinduksi Streptozotocin, Universitas Ahmad Dahlan.
Sholikha, M., and Wulandari, A., 2022, Tyrosinase Inhibition Activity and Phytochemical Screening of Melaleuca leucadendron L. Leaves, Borneo J. Pharm., 5 (3), .
Loan, N. T. T., Long, D. T., Yen, P. N. D., Hanh, T. T. M., Pham, T. N., and Pham, D. T. N., 2021, Purification process of mangiferin from mangifera indica l. Leaves and evaluation of its bioactivities, Processes, 9 (5), .
Kemenkes R.I, 2017, Farmakope Herbal Indonesia, .
LPPT UGM, 2023, Lembar kerja uji kimia laboratorium pengujian ekstrak valerian dan katu,.
Retnaningtyas, Y., Kristiningrum, N., Renggani, H. D., and Sary, I. P., 2020, Stability Indicating RP-HPLC for Quantification Mangiferin in Extract of Three Species Mango Leaves, Indo. J. Chem. Res., 8 (1), .
RodrÃguez De Luna, S. L., RamÃrez-Garza, R. E., and Serna SaldÃvar, S. O., 2020, Environmentally Friendly Methods for Flavonoid Extraction from Plant Material: Impact of Their Operating Conditions on Yield and Antioxidant Properties, Sci. World J., 2020, .
Acosta, J., Sevilla, I., Salomón, S., Nuevas, L., Romero, A., and Amaro, D., 2016, Determination of mangiferin solubility in solvents used in the biopharmaceutical industry, J. Pharm. Pharmacogn. Res.,.
Purnama, N. S., Hasan, H., and Pakaya, M. S., 2021, Standarisasi Dan Kadar Flavonoid Total Ekstrak Etil Asetat Kulit Batang Nangka (Artocapus heterophylus L), Indones. J. Pharm. Educ., 1 (3), .
Zainab, Z., Sulistyani, N., and Anisaningrum, A., 2016, Penetapan Parameter Standardisasi Non Spesifik Dan Spesifik Ekstrak Daun Pacar Kuku (Lawsonia inermis L.), Media Farm. J. Ilmu Farm., 13 (2), .
He, M., Zheng, W., Wang, N., Gao, H., Ouyang, D., and Huang, Z., 2022, Molecular Dynamics Simulation of Drug Solubilization Behavior in Surfactant and Cosolvent Injections, Pharmaceutics, 14 (11), .
Sarkar, R., Pal, A., Rakshit, A., and Saha, B., 2021, Properties and applications of amphoteric surfactant: A concise review, J. Surfactants Deterg., 24 (5), .
Shraim, A. M., Ahmed, T. A., Rahman, M. M., and Hijji, Y. M., 2021, Determination of total flavonoid content by aluminum chloride assay: A critical evaluation, LWT, 150, .
Vigneshwari, R., and Dash, S., 2023, Comparative Interaction of Flavonoid Quercetin with Different Tween Surfactants, ACS Food Sci. Technol.,.
Ybañez-Julca, R. O., Asunción-Alvarez, D., Quispe-DÃaz, I. M., Palacios, J., Bórquez, J., Simirgiotis, M. J., Perveen, S., Nwokocha, C. R., Cifuentes, F., and Paredes, A., 2020, Metabolomic Profiling of Mango (Mangifera indica Linn) Leaf Extract and Its Intestinal Protective Effect and Antioxidant Activity in Different Biological Models, Molecules, 25 (21), .
Suliga, J., and Åšliwa, P., 2017, Micellar-mediated extraction of green tea containing Chrysanthemum morifolium flowers, Sci. Technol. Innov., 1 (1), .
Smail, S. S., Ghareeb, M. M., Omer, H. K., Al-Kinani, A. A., and Alany, R. G., 2021, Studies on surfactants, cosurfactants, and oils for prospective use in formulation of ketorolac tromethamine ophthalmic nanoemulsions, Pharmaceutics, 13 (4), .
Miller, R., 2015, Emulsifiers: Types and Uses, in Encyclopedia of Food and Health,.
Shakeel, F., Haq, N., Alanazi, F. K., and Alsarra, I. A., 2017, Solubility and thermodynamic function of apremilast in different (Transcutol + water) cosolvent mixtures: Measurement, correlation and molecular interactions, J. Ind. Eng. Chem., 56, .
Ma, B. L., Yang, Y., Dai, Y., Li, Q., Lin, G., and Ma, Y. M., 2017, Polyethylene glycol 400 (PEG400) affects the systemic exposure of oral drugs based on multiple mechanisms: taking berberine as an example, RSC Adv., 7 (5), .
Huamán-Castilla, N. L., Mariotti-Celis, M. S., MartÃnez-Cifuentes, M., and Pérez-Correa, J. R., 2020, Glycerol as alternative co-solvent for water extraction of polyphenols from Carménère pomace: Hot pressurized liquid extraction and computational chemistry calculations, Biomolecules, 10 (3), .
Niaz, K., and Khan, F., 2020, Analysis of Polyphenolics, in Recent Advances in Natural Products Analysis,.