Inhibition of Edible Plant Torch Ginger (Etlingera elatior (Jack) R. M. Sm.) against α-Glucosidase and α-Amylase

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Published: Dec 29, 2023
DOI: https://doi.org/10.25077/jsfk.10.3.348-352.2023
Keywords:
torch ginger, type-2 diabetes mellitus, anti-diabetes TLC-autography

Main Article Content

Nova Syafni
Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatra, Indonesia. Sumatran Biota Laboratory, Universitas Andalas, Padang, West Sumatra, Indonesia
Athallah Fakhriadi
Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatra, Indonesia.
Nofrizal Nofrizal
Sumatran Biota Laboratory, Universitas Andalas, Padang, West Sumatra, Indonesia. Department of Pharmacy, Dharma Andalas University, Padang, West Sumatra, Indonesia
Friardi Ismed
Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatra, Indonesia. Sumatran Biota Laboratory, Universitas Andalas, Padang, West Sumatra, Indonesia
Deddi Prima Putra
Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatra, Indonesia. Sumatran Biota Laboratory, Universitas Andalas, Padang, West Sumatra, Indonesia

Abstract

Indonesia's diabetes cases were in the top ten list globally, with 90% of the patients being type-2 diabetes mellitus (T2DM).  An approach for screening the local edible plants is made for managing or treating T2DM by inhibiting α-glucosidase and α-amylase enzymes. Results showed that inflorescence of Etlingera elatior inhibition toward both enzymes. TLC-autography and IC50 value of fractions from E. elatior were used to identify the potential fractions and possible compounds for the activity. The non-polar fraction was spotted in the active substances based on TLC-autography. Then, the targeted compounds were separated by column chromatography to obtain stigmasterol as one of the active compounds. The IC50 values of total extract, n-hexane, EtOAc, BuOH, and isolated compound against α-glucosidase were 16.0, 7.5, 13.5, 9.7, and 2.0 ppm, respectively. The IC50 values for α-amylase inhibition were respectively 88.6, 48.6, 23.2, 29.1, and 27.5 ppm. The positive control (acarbose) against α-glucosidase and amylase exhibited IC50 values of 153.2 and 12.3 ppm. The inhibition of E. elatior against the two enzymes could be an alternative to delay carbohydrate absorption.

Article Details

How to Cite
Syafni, N., Fakhriadi, A., Nofrizal, N., Ismed, F., & Putra, D. P. (2023). Inhibition of Edible Plant Torch Ginger (Etlingera elatior (Jack) R. M. Sm.) against α-Glucosidase and α-Amylase. Jurnal Sains Farmasi & Klinis, 10(3), 348–352. https://doi.org/10.25077/jsfk.10.3.348-352.2023
Issue
Vol. 10 No. 3 (2023): J Sains Farm Klin 10(3), Desember 2023
Section
Research Articles
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