Thin layer chromatography (TLC) analysis and antihyperuricemic activity of bamboo shoots extract {Schizostachyum brachycladum Kurz (Kurz)} in male white mice
Article Sidebar
Main Article Content
Abstract
Thin-layer chromatography (TLC) analysis of bamboo shoot extract (Schizostachyum brachycladum) and its antihyperuricemic activity evaluation on male mice have been conducted. The extraction was processed by the maceration method, while the determination of the compound was carried out by using various color reagents of TLC plate. To evaluate the antihyperuricemic activity of the extract, a total of 25 male mice were hyperuricemic-induced with daily chicken liver homogenates along with extract suspension at doses of 25, 50 and 100 mg/kg, allopurinol 10 mg/kg, and control group. Another group consisted of 5 mice was treated as the normal group. Serum uric acid level of mice was measured by using the spectrophotometer at the 8th day and analyzed with one-way ANOVA. The TLC analysis showed that the extract contained phenolic compounds and triterpenoids. The measurement of serum uric acid levels revealed that the extract at doses of 25, 50 and 100 mg/kg significantly decreased the uric acid levels of male white mice as compared with control (p<0.05). However, all doses did not show any significant difference in reducing the uric acid level (p>0.05). The study concluded that bamboo shoot extract exhibited antihyperuricemic activity. The effect was seen even at the lowest dose evaluated.Â
Article Details
The authors retain the copyright and grant the journal the right of first publication simultaneously under the Creative Commons Attribution License. This license allows others to share the work with proper acknowledgment of authorship and initial publication in this journal. Authors are permitted and encouraged to deposit their articles in institutional repositories, on their personal websites, or in other online repositories after the article has been published in JSFK.
References
Bystriakova, N., Kapos, V., Lysenko, I., & Stapleton, C. M. A. (2003). Distribution and conservation status of forest bamboo biodiversity in the Asia-Pacific Region. Biodiversity and conservation, 12(9), 1833-1841.
Widjaya, E.A. (2004). Keanekaragaman Bambu di Pulau Sumba. Puslitbang Biologi. Bogor: LIPI Dewi, R. E. K. (2008). Evaluasi Koleksi Pada Perpustakaan Pusat Penelitian Biologi-Lipi Dalam Menunjang Kebutuhan Informasi Bagi Peneliti: Kajian Analisis Sitiran Pada Karya Penelitian Bidang Botani.
Choudhury, D., Sahu, J. K., & Sharma, G. D. (2012). Value addition to bamboo shoots: a review. Journal of Food Science and Technology, 49(4), 407-414.
Puri, H. S. (2003). Rasayana: Ayurvedic herbs for longevity and rejuvenation. CRC Press.
Hawkins, D. W., & Rahn, D. W. (2005). Gout and hyperuricemia. Pharmacotherapy, A pathophysiological Approach, McGraw-Hill.
Hayden, M. R., & Tyagi, S. C. (2004). Uric acid: A new look at an old risk marker for cardiovascular disease, metabolic syndrome, and type 2 diabetes mellitus: The urate redox shuttle. Nutrition & Metabolism, 1(1), 10.
Smith, E., & March, L. (2015). Global prevalence of hyperuricemia: a systematic review of population-based epidemiological studies. Arthritis & Rheumatology, 67, 2690-2692.
Wallace, K. L., Riedel, A. A., Joseph-Ridge, N., & Wortmann, R. (2004). Increasing prevalence of gout and hyperuricemia over 10 years among older adults in a managed care population. The Journal of rheumatology, 31(8), 1582-1587.
Pacher, P., Nivorozhkin, A., & Szabó, C. (2006). Therapeutic effects of xanthine oxidase inhibitors: renaissance half a century after the discovery of allopurinol. Pharmacological Reviews, 58(1), 87-114.
Widyaningsih, W. (2011). Efek Ekstrak Etanol Herba Putri Malu (Mimosa pudica, L) Sebagai Penrunan Kadar Asam Urat Serum Mencit Jantan Galur Swiss. Prosiding Seminar Nasional "Home Care", Universitas Ahmad Dahlan.
Stahl, E. (2013). Thin-Layer Chromatography: A Laboratory Handbook. Springer
Harbone, J. B. (1987). Metode fitokimia penuntun cara modern menganalisa tumbuhan. Cetakan II, Diterjemahkan oleh K. Padawinata dan I. Soediro. Bandung: ITB.
Wagner, H., & Bladt, S. (1996). Plant drug analysis: a thin layer chromatography atlas. Springer Science & Business Media.
Hamzah, L., Arifin, H., & Ahmad, A. (2014). Pengaruh Ekstrak Etanol Rambut Jagung (Zea Mays L.) Terhadap Kadar Asam Urat Darah Mencit Putih Jantan Hiperurisemia.
Bourne dan Zastrow. (2001). Reseptor dan Farmakdinamika Obat. Dalam: Farmakologi Dasar dan Klinik. Editor: Katzung, B.G. Penerjemah: Dripa Sjabana. Buku I. Edisi 8. Jakarta: Penerbit Salemba Medika.
Tarigan, I. M., Bahri, S., & Saragih, A. (2013). Aktivitas Antihiperurisemia Ekstrak Etanol Herba Suruhan (Peperomia pellucida (L.) Kunth) pada Mencit Jantan. Journal of Pharmaceutics and Pharmacology, 1(1), 37-43.
Azmi, S. M. N., Jamal, P., & Amid, A. (2012). Xanthine oxidase inhibitory activity from potential Malaysian medicinal plant as remedies for gout. Int Food Res J, 19(1), 159-65.
Moriwaki, Y., Okuda, C., Yamamoto, A., Ka, T., Tsutsumi, Z., Takahashi, S., ... & Wakame, K. (2011). Effects of Oligonol®, an oligomerized polyphenol formulated from lychee fruit, on serum concentration and urinary excretion of uric acid. Journal of Functional Foods, 3(1), 13-16.