Activities of Red Spinach (Amaranthus tricolor L.) Ethanolic Extract in Steatosis Prevention: in Vivo Study
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Abstract
This study aimed to examine the activities of red spinach (Amaranthus tricolor L.) ethanolic extract in preventing steatosis by referring to ALT activities and liver histopathological parameters. Thirty male Sprague-Dawley rats were divided into 6 groups of normal, negative control, positive control (0.9 mg/kgBW simvastatin), treatment I (200 mg/kgBW extract), treatment II (400 mg/kgBW extract), and treatment III (800 mg/kgBW extract). From day 1 to day 67, apart from the normal and negative-control groups, all other groups were given preventive therapy, and high-fat, high-cholesterol diet was applied two hours after preventive therapy from day 8 to day 67. ALT parameters were determined on day 0 and 67. Histopathological changes in the liver were examined on day 68. One-way ANOVA was performed followed by post-hoc Tukey HSD test. The results indicated that the ethanolic extract of red spinach at doses of 200 mg/kgBW, 400 mg/kgBW and 800 mg/kgBW could maintain ALT levels at normal values. Histopathological image of the liver treated with ethanolic extract of red spinach at doses of 200 mg/kgBW, 400 mg/kgBW, and 800 mg/kgBW showed normal condition. These findings suggest that standardized ethanol extract of red spinach leaves has the potential to prevent steatosis.
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References
Al Mahtab, M., & Akbar, S. M. F. (2013). Non-alcoholic Fatty Liver Disease: A Review. Journal of Gastroenterology and Hepatology Research, 2(3), 439-444.
Sudoyo, A., Bambang, S., Idrus, A., Marcellus, S., Siti, S., 2009. Buku Ajar Ilmu Penyakit Dalam Jilid I Edisi V. Jakarta: Interna Publishing.
Dabhi, A. S., Brahmbhatt, K. J., Pandya, T. P., Thorat, P. B., & Shah, M. C. (2008). Non-alcoholic fatty liver disease (NAFLD). Journal Indian Academy of Clinical Medicine, 9(1), 36-41.
Wiranto, W.P., (2004). Memanfaatkan tanaman sayur untuk mengatasi aneka penyakit. Depok: AgroMedia Pustaka.
Samsul, A., Krupanidhi, K., & Sambasiva Rao, K. R. S. (2013). Evaluation of in-vitro antioxidant activity of Amaranthus tricolor Linn. Asian Journal of Pharmacology and Toxicology, 1(01), 12-16.
Clemente, A., & Desai, P. V. (2011). Evaluation of the hematological, hypoglycemic, hypolipidemic and antioxidant properties of Amaranthus tricolor leaf extract in rat. Tropical Journal of Pharmaceutical Research, 10(5), 595-602.
Pradana, D. A., Anggriani, I. D., & Setyaningrum, T. R. (2016). Potential of Red Spinach Leaves Ethanolic Extract (Amaranthus tricolor L.) as a Complementary Therapy For Hiperlipidemia: Study in Vivo of Histopathologic and Activity of Alanin Aminotransferase (ALT). Jurnal Sains Farmasi & Klinis, 3(1), 6-13.
Pradana, D. A., Rahmah, F. S., & Setyaningrum, T. R. (2016). Potensi Antihiperlipidemia Ekstrak Etanolik Daun Bayam Merah (Amaranthus tricolor L.) Terstandar secara in Vivo Berdasarkan Parameter LDL (Low Density Lipoprotein). Jurnal Sains Farmasi & Klinis, 2(2), 122-128.
Pradana, D.A., Pondawinata M., Widyarini, S.., (2017). Red spinach (Amaranthus tricolor L.) ethanolic extract as prevention against atherosclerosis based on the level of Low-Density Lipoprotein and histopathological feature of aorta in male Sprague-Dawley rats, AIP Conference Proceedings (https://dx.doi.org/10.1063/1.4978152) (diakses 20 April 2017)
Reference Values for Laboratory Animals, Research Animal Resources, University of Minnesota. www.ahc.umn.edu/rar/refvalues.html (diakses 20 Mei 2016)
Junqueri, L., Carnerio, J., (2007). Histologi dasar : Teks dan atlas. 10th ed. Tambayong J, editor. Jakarta: Buku Kedokteran EGC.
Nasution, A. Y., Adi, P., & Santosa, P. A. (2016). Pengaruh Ekstrak Propolis terhadap Kadar SGOT (Serum Glutamic Oxaloacetic Transaminase) dan SGPT (Serum Glutamic Pyruvic Transaminase) pada Tikus Putih (Rattus norvegicus) Galur Wistar dengan Diet Tinggi Lemak. Majalah Kesehatan FKUB, 2(3), 120-126.
Kumar V, F.N., (2005). Cellular adaptations, cell injury, and cell death. Dalam: Kumar V, Abbas AK, Fausto N, penyunting. Robbins and Cotran pathologic basis of disease. Ed 7. Philadelphia: Elsevier Saunders; hlm.5-21.
Kuntz, E., (2006). Hepatology, principles and practice. Edisi ke-2. Wetzlar: Springer Medizin Verlag Heidelberg.
Salt, W. B. (2004). Nonalcoholic fatty liver disease (NAFLD): a comprehensive review. Journal of Insurance Medicine, 36(1), 27-41.
Bentz, A. B. (2009). A review of quercetin: Chemistry, antioxidant properties, and bioavailability. Journal of young investigators, 19(10).
Asmariani, W., Probosari, E., 2012. Pengaruh pemberian buah pepaya (Carica papaya L.) terhadap kadar kolesterol LDL dan kolesterol HDL pada tikus Sprague Dawley dengan hiperkolesterolemia. Journal of Nutriton Collage, 1(1), 256-268.