Antibiotic Compound of Symbiotic Bacillus sp1 (HA1) from Marine Sponge Haliclona fascigera
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Abstract
Symbiotic bacteria are bacteria that live within a host tissue and do not harm the host itself. These bacteria can produce various bioactive compounds such as antibacterial compounds. This study aimed to isolate the antibiotic compounds from ethyl acetate extract of symbiotic bacteria Bacillus sp.1 (HA1) of marine sponge Haliclona fascigera. Bacterial cultivation was performed on Nutrient Broth medium using incubator shaker at 150 rpm at 37ºC for 24 hours. The isolation of antibiotic compound was done by column chromatography method and testing of antibacterial activity was conducted by agar diffusion method. The study revealed 2 pure compounds W1 and W2. The compound W1 (10.8 mg) was soluble in the ethyl acetate and methanol but was difficult to dissolve in n-hexane. This compound could inhibit the growth of pathogenic bacteria Staphylococcus aureus ATCC 25923 with 1% of minimum inhibition concentration (MIC). While the compound W2 (2.3 mg) was soluble in dichloromethane and methanol but was difficult to dissolve in n-hexane. The W2 compound could not inhibit the growth of Staphylococcus aureus ATCC 25923.
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