Diferensiasi Gelatin Sapi dan Babi pada Cangkang Kapsul Keras Menggunakan metode Kombinasi Kromatografi Cair Kinerja Tinggi dan Kemometrik

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Published: Oct 24, 2022
DOI: https://doi.org/10.25077/jsfk.9.2.88-94.2022
Keywords:
diferensiasi, gelatin babi, gelatin sapi, KCKT, PCA

Main Article Content

Zilhadia Zilhadia
Program Studi Farmasi Fakultas Ilmu Kesehatan UIN Syarif Hidayatullah Jakarta
Fathmah Syafiqoh
Program Studi Farmasi Fakultas Ilmu Kesehatan UIN Syarif Hidayatullah Jakarta
Ofa Suzanti Betha
Program Studi Farmasi Fakultas Ilmu Kesehatan UIN Syarif Hidayatullah Jakarta

Abstract

Gelatin sapi dan babi merupakan bahan utama pembuatan cangkang kapsul keras. Gelatin babi tidak boleh dikonsumsi oleh Muslim sehingga perlu dilakukan analisis pembeda gelatin sapi dan babi. Tujuan penelitian ini adalah untuk mendiferensiasi gelatin sapi dan babi pada cangkang kapsul keras menggunakan metode kombinasi Kromatografi Cair Kinerja Tinggi (KCKT) dan Kemometrik menu Principal Komponen Analisis(PCA). Gelatin di ekstraksi dari cangkang kapsul keras dan langsung dihidrolisis menggunakan teknik hidrolisis asam, diinjeksikan ke dalam alat KCKT dan tinggi puncak kromatogram setiap asam amino penyusun gelatin dianalisis. Hasil penelitian menunjukkan bahwa asam amino penyusun gelatin dapat dipisahkan dengan baik oleh KCKT. Gelatin standar dan gelatin dari cangkang kapsul dengan sumber hewan yang sama memiliki komposisi asam amino yang sama. Dengan demikian, PCA dapat mengklasifikasikan sumber gelatin pada cangkang kapsul simulasi. Namun penelitian ini belum berhasil mengidentifikasi sumber gelatin cangkang kapsul komersial 

Article Details

How to Cite
Zilhadia, Z., Syafiqoh, F., & Betha, O. S. (2022). Diferensiasi Gelatin Sapi dan Babi pada Cangkang Kapsul Keras Menggunakan metode Kombinasi Kromatografi Cair Kinerja Tinggi dan Kemometrik. Jurnal Sains Farmasi & Klinis, 9(2), 88–94. https://doi.org/10.25077/jsfk.9.2.88-94.2022
Issue
Vol. 9 No. 2 (2022): J Sains Farm Klin 9(2), Agustus 2022
Section
Research Articles
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Author Biography

Zilhadia Zilhadia, Program Studi Farmasi Fakultas Ilmu Kesehatan UIN Syarif Hidayatullah Jakarta

Scholar ID: https://scholar.google.co.id/citations?user=TxPUWOvuPqsC&hl=id&oi=ao

 

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