Identification of the type of human papillomavirus (HPV) in patients with cervical cancer
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Abstract
Human Papillomavirus (HPV) is the most significant risk factor for the cause of cervical cancer. The purpose of this study for identification of HPV types 16, 18, 31, 33, 45 and 52 in cervical cancer patients. HPV is a row of high-risk HPV types that can cause cervical cancer. Total sample of 78 diisolat DNA derived from FFPE, cervical smears and cervical cancer fresh tissue obtained from Dr. Dr. M. Djamil, Padang and hospitals. Arifin Achmad, Pekanbaru. HPV DNA detection is done by using Polymerase Chain Reaction (PCR) using universal primers GP5 +/6 +. HPV types were identified by PCR with specific primers. Total sample types obtained with concentrations varying between 0.9 to 645 ng / ml with purity DNA in accordance with the specified purity for PCR amplification. The results of the study of 78 patients with cervical cancer samples, 42 samples (54%) identified HPV DNA. HPV type 18 is more dominant and followed by HPV type 16 as compared to the other types, namely the percentage of 40.4% and 28.5%. HPV type 45 (7.1%), HPV type 52 (2.3%) and HPV 31 and HPV type 33 was not detected.
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References
Bernard HU. (2004). The clinical importance of the nomenclatur, evolution, and taxonomy of human papillomaviruses. Clin Virol (32),1-6.
Bruni, L., Barrionuevo-Rosas, L., Serrano, B., Brotons, M., et al. (2014). Human papillomavirus and Related diseases report in Indonesia. Barcelona : ICO Information Center on HPVand Cancer.
Calvagna, M. (2007). Diagnosis of Cervical Cancer. American Cancer Society website.http://www.cancer.org (12 Januari 2015).
Conway, M.J., & Meyers C. (2009). Replication and Assembly of Human Papillomaviruses. J Dent Res, 88 (4): 307-317.
Kepmenkes. (2013). Pusat Data dan Informasi, Badan Penelitian dan Pengembangan Kesehatan Kementrian Kesehatan Republik Indonesia.
Munoz, N., Bosch, F.X., de Sanjose, S. (2003). Epidemiologic classification of Human papillomavirus types associated with cervical cancer. The New Engl J Med, 348(6), 518-27.
Paavonen J. (2007). Human Papillomavirus Infection and the Development of Cervical Cancer and Related Genital Neoplasia. International Journal of Infectious Disease, 11(Suplement 2): 5359.
Parkin, D,. M & Bray, F. (2006). The burden of HPV-related cancers. Vaccine, 24,11-25.
Rizkiany, H. N. (2013). Identifikasi karakter aromatik berdasarkan PCR dan organoleptik BC3F2 ciherang x mentikwangi. (Skripsi). Bogor: Institut Pertanian Bogor.
Sambrook, J., Russel, D.W. (1989). Molecular cloning, A Laboratory manual 2nd Edition. New York: Cold-spring Harbour Laboratory Press.
Thermo Scientific. (2011). Assesment of nucleic acid purity. T042-Technical Bulletin Nano Drop Spectrophotometers. New York: Wilmington
Venuti, A., Paolini, F., Nasir, L., Corteggio, A., Roperto, S., Campo, M. S., & Borzacchiello, G. (2011). Papillomavirus E5: the smallest oncoprotein with many functions. Molecular Cancer, 10(1), 1.