Waktu Kultivasi Optimal dan Aktivitas Antibakteri dari Ekstrak Etil Asetat Jamur Simbion Aspergillus unguis (WR8) dengan Haliclona fascigera

Agnes Rendowaty, Akmal Djamaan, Dian Handayani

Abstrak


Penelitian tentang penentuan waktu optimal pertumbuhan jamur simbion Aspergillus unguis (WR8) dan pengujian aktivitas antibakteri telah dilakukan. Kultivasi jamur dilakukan dengan menggunakan media Sabaoraud Dextrose Broth (SDB) dalam keadaan statis dan di-shaker (120 rpm) pada suhu 25-28oC. Pertumbuhan jamur secara optimal ditentukan berdasarkan jumlah biomassa kering miselia jamur per satuan waktu. Media cair diekstraksi dengan pelarut etil asetat, ekstrak etil asetat selanjutnya digunakan untuk pengujian aktivitas antibakteri menggunakan metode difusi agar terhadap bakteri Staphylococcus aureus. Hasil penelitian menunjukkan bahwa waktu optimal pertumbuhan jamur simbion tersebut dalam keadaan statis adalah pada hari ke-21 dan diameter hambat ekstrak (5 %b/v) adalah 21 mm. Sedangkan waktu optimal pertumbuhan jamur yang di shaker adalah pada hari ke-14 dan diameter hambat ekstrak sebesar 11 mm. Dapat disimpulkan waktu kultivasi optimal untuk menghasilkan senyawa antibakteri dari jamur A. unguis (WR8) adalah selama 21 hari secara statis menggunakan media cair.  


Kata Kunci


jamur simbion; Aspergillus unguis; waktu kultivasi; aktivitas antibakteri; Haliclona fascigera.

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Referensi


Mariottini, G.L., & Grice, I. D. (2016). Antimicrobials from Cnidarians: A New Perspective for Anti-Infective Theraphy?, Marine Drugs, 14(48), 1–19.

Xu, L., Meng, W., Cao, C., Wang, J., Shan, W., & Wang, Q. (2015). Antibacterial and Antifungal Compounds from Marine Fungi-Review. Marine Drugs, 13(6), 3479–3513.

Pruksakorn, P., Arai, M., Kotoku, N., Vilchèze, C., Baughn, A. D., Moodley, P., …Kobayashi, M. (2010). Trichoderins, novel aminolipopeptides from a marine sponge-derived Trichoderma sp., are active against dormant mycobacteria. Bioorganic & Medicinal Chemistry Letters, 20(12), 3658–3663

Wang, G. Y. S., Abrell, L. M., Avelar, A., Borgeson, B. M., & Crews, P. (1998). New Hirsutane Based Sesquiterpenes from Salt Water Cultures of a Marine Sponge-Derived Fungus and the Terrestrial Fungus. Tetrahedron, 54(26), 7335–7342

Handayani, D., Ahdinur. R. F., & Rustini, R. (2015). Antimicrobial Activity of Endophytic Fungi from Marine Sponge Haliclona fascigera. Journal of Applied Pharmaceutical Science, 5(10), 154–156.

Rasyid, W. (2015). Penapisan Aktivitas Sitotoksis Ekstrak Etil Asetat Jamur Simbion dari Spon Laut Haliclona fascigera dengan Metode Brine Shrimp Lethality Test (BSLT). Skripsi. Fakultas Farmasi. Universitas Andalas.

Vandermolen, K. M., Raja, H. A., El-Elimat, T., & Oberlies, N. H. (2013). Evaluation of culture media for the production of secondary metabolites in a natural products screening program. AMB Express, A Springer Open Journal, 3(71), 1–7

Derakhshan, A., Rabindra, R. J., Ramanujam, B., & Rahimi, M. Evaluation of Different Media and Methods of Cultivation Fungi, Verticillium lecabii (zimm.) Viegas. Pakistan Journal of Biological Sciences, 11(11), 1506–1509.

Basu, S., Bose, C., Ojha, N., Das, N., Pal, M., & Khurana, S. (2015). Evolution of Bacterial and Fungal Growth Media. Bioinformation, 11(4). 182–184.

Tan, Y. P., & Chan, E. W. C. (2014). Antioxidant, antityrosinase and Antibacterial Properties of Fresh and Processed Leaves of Anacardium occidentale and Piper betle. Food Bioscience, 6(6), 17–23.

Eltem, R., Askun, T., Sarigul, N., Taskin, E. O., & Efendler, H. (2004). Colonial and Morphological Characteristics of Some Aspergillus Fr:Fr. Species Isolated from Vineyards in Manisa and Azmir Provinces (turkey). Turkish Journal of Botany, 28(3), 287–298.

Maier, R. M., (2000). Bacterial Growth, In Review of Basic Microbiological Concepts (pp 37–55). Academic Press.

Nursid, M., Manulang, M., Samiadji, J., & Maraskuranto, E. (2015). Effect of Agitation Speed and Cultivation Time on The Production of The Emestrin Produced by Emericella nidulans Marine Fungal. Squalen Buletinl of Marine & Fisheries Postharvest and Biotechnology, 10(2), 73–78.

Ruiz, B., Chavez, A., Forero, A., Garcia-Huante, Y., Romero, A., Sanchez, M., Rocha, D., ... & Langley, E. (2010). Production of Microbial Secondary Metabolites: Regulation by the carbon source. Critical Review in Microbiology, 36(2), 146–167.

Gonzalez, J. B., Fernandez, F. J., & Tomasini, A. (2003). Microbial Secondary Metabolites Production and Strain Improvement. Indian Jurnal of Biotechnology, 2(3), 322–333.

Kawahara, N., Nakajima, S., Satoh, Y., Yamazaki, M., & Ken-Ichi Kawai. (1988). Studies on Fungal Products. XVIII. Isolation and Structure of a New Fungal Depsidone Relted to Nidulin and a New Phthalide from Emericella unguis. Chemical and Pharmaceutical Bulletin, 36(6), 1970–1975

Kawahara, N., Nozawa, K., Nakajima, S., Kawai, K., & Yamazaki, M. (1988). Isolation and Structures of Novel Fungal Depsidones Emeguisins A,B, and C, from Emericella unguis. Journal of Chemical Society Perkin Trans, 1(9), 2611–2614

Hamano, K., Kinoshita-Okami, M., Hemmi, A., Sato, A., Hisamoto, M., Matsuda, K., … Kazuhiko Tanzawa. (1992). Folipasatin, A New Depsidone Compound from Aspergillus unguis as an inhibitor of Phispholipase A2. Jurnal Antibiotics, 45(8), 1195–1201

Nielsen, J., Nielsen, P. H., & Frisvad, J. C. (1999). Fungal depside, guisinol, from a marine derived strain of Emericella unguis. Phytochemistry, 50(2), 263–265

Malmstrøm, J., Ryager, A., Anthoni, U., & Nielsen, P. H. (2002). Unguisin C, a GABA-containing cyclic peptide from the fungus Emericella unguis. Phytochemistry, 60(8), 869–872

Sureram, S., Kesornpun, C., Mahidol, C., Ruchirawat, S., & Kittakoop, P. (2013). Directed biosynthesis through biohalogenation of secondary metabolites of the marine-derived fungus Aspergillus unguis. RSC Advances, 3(6), 1781–1788.

Sureram, S., Wijakrutta, S., Ngamrojanavanich, N., Mahidol, C., Ruchirawat, S., & Kittakoop, P. (2012). Depsidones, Aromatase Inhibitors and Radical Scavenging Agents from the Marine-Derived Fungus Aspergillus unguis CRI282-03. Planta Medica, 78(6), 582–588.




DOI: https://doi.org/10.29208/jsfk.2017.4.1.147

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